Protein Variants | Comment | Organism |
---|---|---|
H225Q | modest changes in kinetic parameters, no stabilization of FADH2-iminium charge-transfer complex | Arthrobacter globiformis |
Y259F | able to oxidize substrate, steady-state turnover is attenuated 200fold, rate of FAD reduction is substantially impaired, no stabilization of FADH2-iminium charge-transfer complex | Arthrobacter globiformis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.041 | - |
O2 | wild-type, pH 8.5, 25°C | Arthrobacter globiformis | |
0.117 | - |
O2 | mutant H225Q, pH 8.5, 25°C | Arthrobacter globiformis | |
2.4 | - |
N,N-dimethylglycine | wild-type, pH 8.5, 25°C | Arthrobacter globiformis | |
47 | - |
N,N-dimethylglycine | mutant Y259F, pH 8.5, 25°C | Arthrobacter globiformis | |
142 | - |
N,N-dimethylglycine | mutant H225Q, pH 8.5, 25°C | Arthrobacter globiformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter globiformis | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
N,N-dimethylglycine + H2O + O2 | - |
Arthrobacter globiformis | sarcosine + formaldehyde + H2O2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.047 | - |
N,N-dimethylglycine | mutant Y259F, pH 8.5, 25°C | Arthrobacter globiformis | |
10.6 | - |
N,N-dimethylglycine | wild-type, pH 8.5, 25°C | Arthrobacter globiformis | |
13.6 | - |
N,N-dimethylglycine | mutant H225Q, pH 8.5, 25°C | Arthrobacter globiformis |