Literature summary for 1.5.3.1 extracted from

Tong, Y.; Xin, Y.; Yang, H.; Zhang, L.; Tao, X.; Xu, H.; Wang, W.
Novel affinity purification of monomeric sarcosine oxidase expressed in Escherichia coli (2013), J. Sep. Sci., 36, 3086-3092 .

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Bacillus sp. BSD-8

Organism

Organism	UniProt	Comment	Textmining
Bacillus sp. BSD-8	Q6ITC6	-	-

Purification (Commentary)

Purification (Comment)	Organism
affinity-purification, 4-aminopyrrole-2-carboxylic acid as the affinity ligand, which is coupled with Sepharose CL 4B via spacers composed of epichlorohydrin and ethylenediamine. With the affinity medium, the purification process consists of only one affinity chromatography step to capture monomeric enzyme. The purified enzyme iss 94% and 96% pure when analyzed on an HPLC and reducing SDS-PAGE	Bacillus sp. BSD-8

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
sarcosine + H2O + O2	-	Bacillus sp. BSD-8	glycine + formaldehyde + H2O2	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 46000, SDS-PAGE	Bacillus sp. BSD-8

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Bacillus sp. BSD-8

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Release 2023.1 (January 2023)