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Literature summary for 1.5.3.1 extracted from

  • Saito, M.; Kanno, M.; Iizuka, H.; Suzuki, H.
    Kinetic studies on the role of Lys-171 and Lys-358 in the beta subunit of sarcosine oxidase from Corynebacterium sp. U-96 (2007), J. Biochem., 141, 799-815.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
mutant proteins expressed in Escherichia coli BL21(DE3) Corynebacterium sp. U-96

Protein Variants

Protein Variants Comment Organism
K171A 39% activity of the wild-type enzyme, strongly increased Km for oxygen Corynebacterium sp. U-96
K171D 32% activity of the wild-type enzyme, strongly increased Km for oxygen Corynebacterium sp. U-96
K171R 58% activity of the wild-type enzyme Corynebacterium sp. U-96
K358A inactive Corynebacterium sp. U-96
K358D inactive Corynebacterium sp. U-96
K358R 0.07% of activity, increased Km Corynebacterium sp. U-96

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0312
-
oxygen K171R mutant enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
0.0329
-
oxygen wild-type enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
0.386
-
oxygen K171A mutant enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
0.73
-
oxygen K171D mutant enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
1.47
-
sarcosine K171A mutant enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
1.72
-
sarcosine K171R mutant enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
2.75
-
sarcosine K171D mutant enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
2.76
-
sarcosine wild-type enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
17.4
-
sarcosine K358R mutant enzyme, pH 8.0, 25°C Corynebacterium sp. U-96

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sarcosine + O2 + H2O Corynebacterium sp. U-96
-
formaldehyde + glycine + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Corynebacterium sp. U-96
-
-
-

Reaction

Reaction Comment Organism Reaction ID
sarcosine + H2O + O2 = glycine + formaldehyde + H2O2 mechanism suggested Corynebacterium sp. U-96

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sarcosine + O2 + H2O
-
Corynebacterium sp. U-96 formaldehyde + glycine + H2O2
-
?

Synonyms

Synonyms Comment Organism
monomeric sarcosine oxidase
-
Corynebacterium sp. U-96
MSOX
-
Corynebacterium sp. U-96

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0113
-
sarcosine K358R mutant enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
2.25
-
sarcosine K171A mutant enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
2.64
-
sarcosine K171D mutant enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
6.1
-
oxygen K171D mutant enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
7.4
-
oxygen K171A mutant enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
9.23
-
sarcosine K171R mutant enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
9.8
-
oxygen K171R mutant enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
18.5
-
sarcosine wild-type enzyme, pH 8.0, 25°C Corynebacterium sp. U-96
18.9
-
oxygen wild-type enzyme, pH 8.0, 25°C Corynebacterium sp. U-96

Cofactor

Cofactor Comment Organism Structure
FAD covalent and non-covalent Corynebacterium sp. U-96
FMN covalent Corynebacterium sp. U-96