Literature summary for 1.5.3.1 extracted from

Hassan-Abdallah, A.; Zhao, G.; Chen, Z.W.; Mathews, F.S.; Schuman Jorns, M.
Arginine 49 is a bifunctional residue important in catalysis and biosynthesis of monomeric sarcosine oxidase: a context-sensitive model for the electrostatic impact of arginine to lysine mutations (2008), Biochemistry, 47, 2913-2922.

Search for more literature for 1.5.3.1

Cloned(Commentary)

Cloned (Comment)	Organism
native and mutant enzyme	Bacillus sp. (in: Bacteria)

Crystallization (Commentary)

Crystallization (Comment)	Organism
R49K mutant after reconstitution with FAD, sitting drop vapor diffusion method, high salt or high PEG conditions	Bacillus sp. (in: Bacteria)

Protein Variants

Protein Variants	Comment	Organism
R49K	40fold decrease of turnover, 150fold decrease of catalytic efficiency, structure very similar to wild type enzyme	Bacillus sp. (in: Bacteria)

Inhibitors

Inhibitors	Comment	Organism	Structure
methylthioacetate	-	Bacillus sp. (in: Bacteria)
N-(cyclopropyl)glycin	suicide substrate	Bacillus sp. (in: Bacteria)
pyrrole-2-carboxylate	-	Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.01	-	sarcosine	R49K mutant, pH 8.0, 25°C	Bacillus sp. (in: Bacteria)
0.29	-	sarcosine	native enzyme, pH 8.0, 25°C	Bacillus sp. (in: Bacteria)
7.6	-	sarcosine	native enzyme, pH 8.0, 25°C	Bacillus sp. (in: Bacteria)
28.8	-	sarcosine	R49K mutant, pH 8.0, 25°C	Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
sarcosine + O2 + H2O	Bacillus sp. (in: Bacteria)	-	formaldehyde + glycine + H2O2	-	?

Organism

Organism	UniProt	Comment	Textmining
Bacillus sp. (in: Bacteria)	P40859	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant native and mutant enzyme	Bacillus sp. (in: Bacteria)

Reaction

Reaction	Comment	Organism	Reaction ID
sarcosine + H2O + O2 = glycine + formaldehyde + H2O2	mechanism suggested	Bacillus sp. (in: Bacteria)	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
additional information	also oxidizes other amino acids containing a secondary amino group e.g. L-proline, and N-methyl-L-alanine	Bacillus sp. (in: Bacteria)	?	-	?
sarcosine + O2 + H2O	-	Bacillus sp. (in: Bacteria)	formaldehyde + glycine + H2O2	-	?

Synonyms

Synonyms	Comment	Organism
monomeric sarcosine oxidase	-	Bacillus sp. (in: Bacteria)
MSOX	-	Bacillus sp. (in: Bacteria)

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.7	-	sarcosine	R49K mutant, pH 8.0, 25°C	Bacillus sp. (in: Bacteria)
101	-	sarcosine	native enzyme, pH 8.0, 25°C	Bacillus sp. (in: Bacteria)

Cofactor

Cofactor	Comment	Organism	Structure
FAD	covalently bound	Bacillus sp. (in: Bacteria)

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)