Literature summary for 1.5.1.9 extracted from

Serrano, G.C.; Rezende e Silva Figueira, T.; Kiyota, E.; Zanata, N.; Arruda, P.
Lysine degradation through the saccharopine pathway in bacteria: LKR and SDH in bacteria and its relationship to the plant and animal enzymes (2012), FEBS Lett., 586, 905-911.

Search for more literature for 1.5.1.9

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Nostoc punctiforme
expressed in Escherichia coli BL21(DE3) cells	Ruegeria pomeroyi

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
44000	-	x * 44000, calculated from amino acid sequence	Nostoc punctiforme
44000	-	x * 44000, calculated from amino acid sequence	Ruegeria pomeroyi
44100	-	x * 44100, SDS-PAGE	Nostoc punctiforme
44100	-	x * 44100, SDS-PAGE	Ruegeria pomeroyi

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
saccharopine + NAD+ + H2O	Nostoc punctiforme	-	L-glutamate + alpha-aminoadipate semialdehyde + NADH + H+	-	r
saccharopine + NAD+ + H2O	Ruegeria pomeroyi	-	L-glutamate + alpha-aminoadipate semialdehyde + NADH + H+	-	r
saccharopine + NAD+ + H2O	Nostoc punctiforme ATCC 29133	-	L-glutamate + alpha-aminoadipate semialdehyde + NADH + H+	-	r

Organism

Organism	UniProt	Comment	Textmining
Nostoc punctiforme	-	-	-
Nostoc punctiforme ATCC 29133	-	-	-
Ruegeria pomeroyi	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Q-Sepharose column chromatography, Ni-NTA agarose column chromatography, and Superdex 200 gel filtration	Nostoc punctiforme
Q-Sepharose column chromatography, Ni-NTA agarose column chromatography, and Superdex 200 gel filtration	Ruegeria pomeroyi

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
saccharopine + NAD+ + H2O	-	Nostoc punctiforme	L-glutamate + alpha-aminoadipate semialdehyde + NADH + H+	-	r
saccharopine + NAD+ + H2O	-	Ruegeria pomeroyi	L-glutamate + alpha-aminoadipate semialdehyde + NADH + H+	-	r
saccharopine + NAD+ + H2O	-	Nostoc punctiforme ATCC 29133	L-glutamate + alpha-aminoadipate semialdehyde + NADH + H+	-	r

Subunits

Subunits	Comment	Organism
?	x * 44000, calculated from amino acid sequence	Nostoc punctiforme
?	x * 44000, calculated from amino acid sequence	Ruegeria pomeroyi
?	x * 44100, SDS-PAGE	Nostoc punctiforme
?	x * 44100, SDS-PAGE	Ruegeria pomeroyi

Synonyms

Synonyms	Comment	Organism
saccharopine dehydrogenase	-	Nostoc punctiforme
saccharopine dehydrogenase	-	Ruegeria pomeroyi
SDH	-	Nostoc punctiforme
SDH	-	Ruegeria pomeroyi

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	the enzyme has slightly higher affinity for NAD+ compared to NADP+	Nostoc punctiforme
NAD+	the enzyme has slightly higher affinity for NAD+ compared to NADP+	Ruegeria pomeroyi
NADP+	-	Nostoc punctiforme
NADP+	-	Ruegeria pomeroyi

pI Value

Organism	Comment	pI Value Maximum	pI Value
Nostoc punctiforme	calculated from amino acid sequence	-	5
Ruegeria pomeroyi	calculated from amino acid sequence	-	5

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)