Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.1.9 extracted from

  • Zhu, X.; Tang, G.; Galili, G.
    Characterization of the two saccharopine dehydrogenase isozymes of lysine catabolism encoded by the single composite AtLKR/SDH locus of Arabidopsis (2000), Plant Physiol., 124, 1363-1371.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.5.1.9

Cloned(Commentary)

Cloned (Comment) Organism
in yeast for heterologous expression Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
 saccharopine pH 9, bifunctional enzyme, lysine-ketoglutarate reductase (EC 1. 5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein Arabidopsis thaliana
0.05
-
 saccharopine pH 9, monofunctional enzyme Arabidopsis thaliana
0.063
-
 saccharopine pH 7, bifunctional enzyme, lysine-ketoglutarate reductase (EC 1. 5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein Arabidopsis thaliana
0.13
-
 saccharopine pH 7, monofunctional enzyme Arabidopsis thaliana
0.333
-
 NAD+ pH 7, monofunctional enzyme Arabidopsis thaliana
0.374
-
 NAD+ pH 7, bifunctional enzyme, lysine-ketoglutarate reductase (EC 1. 5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein Arabidopsis thaliana
0.698
-
 NAD+ pH 9, bifunctional enzyme, lysine-ketoglutarate reductase (EC 1. 5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein Arabidopsis thaliana
0.759
-
 NAD+ pH 9, monofunctional enzyme Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Arabidopsis thaliana 5829
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 x * 63000, SDS-PAGE, monofunctional enzyme Arabidopsis thaliana
120000
-
 x * 120000, SDS-PAGE, lysine-ketoglutarate reductase (EC 1. 5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O Arabidopsis thaliana involved in lysine catabolism L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
heterologously expressed enzyme Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O
-
 Arabidopsis thaliana L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
 r
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O involved in lysine catabolism Arabidopsis thaliana L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
 r

Subunits

Subunits Comment Organism
? x * 63000, SDS-PAGE, monofunctional enzyme Arabidopsis thaliana
? x * 120000, SDS-PAGE, lysine-ketoglutarate reductase (EC 1. 5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
AtLKR/SDHp bifunctional enzyme, lysine-ketoglutarate reductase (EC 1. 5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein Arabidopsis thaliana
AtSDHp monofunctional enzyme Arabidopsis thaliana
SDH
-
 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 monofunctional enzyme Arabidopsis thaliana
9.5
-
 bifunctional enzyme, lysine-ketoglutarate reductase (EC 1. 5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Arabidopsis thaliana