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Literature summary for 1.5.1.9 extracted from
- Saccharopine cleavage by a dehydrogenase of human liver (1970), Biochim. Biophys. Acta, 206, 205-214.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| hydroxylamine | slight | Homo sapiens |  |
| NH4Cl | slight | Homo sapiens | |
| p-hydroxymercuribenzoate | - |
Homo sapiens | |
| Sodium bisulfite | slight | Homo sapiens | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.4 | - |
NAD+ | - |
Homo sapiens | |
| 0.5 | - |
saccharopine | - |
Homo sapiens | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | strong inhibition | Homo sapiens | |
| Hg2+ | strong inhibition | Homo sapiens | |
| Zn2+ | strong inhibition | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O | Homo sapiens | involved in lysine catabolism | L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Homo sapiens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Homo sapiens |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, crude enzyme stable for several months, purified enzyme inactivated within 3 weeks | Homo sapiens |
| 2°C, 5-10% loss of activity per week, purified enzyme | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O | N6-(L-1,3-dicarboxypropyl)-L-lysine is identical with saccharopine, high specificity | Homo sapiens | L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+ | - |
r | |
| N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O | involved in lysine catabolism | Homo sapiens | L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+ | - |
r | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Homo sapiens |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 37 | 20°C: 35% of activity maximum, 37°C: 85% of activity maximum | Homo sapiens |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
5 min | Homo sapiens |
| 44 | - |
5 min, 50% activity | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.8 | 9 | - |
Homo sapiens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 9.7 | half maximal activities at pH 7.8 and 9.7 | Homo sapiens |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6.6 | 9.9 | 10 min | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Homo sapiens | |
| NADP+ | 20fold higher activity with NAD+ than NAPD+ | Homo sapiens | |
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