Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.5.1.9 extracted from

  • Fjellstedt, T.A.; Robinson, J.C.
    Properties of partially purified saccharopine dehydrogenase from human placenta (1975), Arch. Biochem. Biophys., 171, 191-196.
    View publication on PubMed
Search for more literature for 1.5.1.9

Inhibitors

Inhibitors Comment Organism Structure
3-acetylpyridine-NAD 15% inhibition Homo sapiens
3-pyridine aldehyde-NAD 40% inhibition Homo sapiens
L-glutamate
-
 Homo sapiens
additional information not: L-lysine-p-nitroanilide Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0645
-
 NAD+
-
 Homo sapiens
1.15
-
 saccharopine
-
 Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
480000
-
 gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O Homo sapiens involved in lysine catabolism L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 not separable from L-lysine-alpha-ketoglutarate reductase
-

Oxidation Stability

Oxidation Stability Organism
5 mM beta-mercaptoethanol preserves loss of activity during enzyme assay Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
placenta
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O
-
 Homo sapiens L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
 r
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O involved in lysine catabolism Homo sapiens L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
 r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 Tris-buffer Homo sapiens
8.9
-
 2-amino-2-methyl-1,3-propanediol buffer Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
7.9 9.3 half maximal activities at pH 7.9 and 9.3 Homo sapiens

pH Stability

pH Stability pH Stability Maximum Comment Organism
8.5
-
 unstable at high pH, beta-mercaptoethanol protects Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Homo sapiens
NADP+ 9% of the rate with NAD+ Homo sapiens