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Literature summary for 1.5.1.9 extracted from

  • Gaziola, S.A.; Teixeira, C.M.; Lugli, J.; Sodek, L.; Azevedo, R.A.
    The enzymology of lysine catabolism in rice seeds. Isolation, characterization, and regulatory properties of a lysine 2-oxoglutarate reductase/saccharopine dehydrogenase bifunctional polypeptide (1997), Eur. J. Biochem., 247, 364-371.
    View publication on PubMed
Search for more literature for 1.5.1.9

Inhibitors

Inhibitors Comment Organism Structure
2-Aminoadipate strong inhibition at 5 mM Oryza sativa
2-oxoglutarate
-
 Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.13
-
 saccharopine
-
 Oryza sativa
0.49
-
 NAD+
-
 Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
202000
-
 native non-denaturing gel, lysine-ketoglutarate reductase (EC 1. 5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein Oryza sativa
203000
-
 gel filtration with Sephacryl S-200, lysine-ketoglutarate reductase (EC 1. 5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein Oryza sativa
396000
-
 native non-denaturing gel, low activity compared to 202000 kDa band Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O Oryza sativa involved in lysine catabolism L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
 rice var IAC-165
-

Purification (Commentary)

Purification (Comment) Organism
lysine-ketoglutarate reductase (EC 1. 5.1.8) and saccharopine dehydrogenase activity (EC 1.5.1.9) occur on a single protein Oryza sativa

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0017
-
 crude extract from immature seeds Oryza sativa
0.052
-
 after purification Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O
-
 Oryza sativa L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
 r
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O involved in lysine catabolism Oryza sativa L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
 r
N6-(L-1,3-dicarboxypropyl)-L-lysine + NAD+ + H2O
-
 Oryza sativa IAC 165 L-glutamate + 2-aminoadipate 6-semialdehyde + NADH + H+
-
 r

Synonyms

Synonyms Comment Organism
SDH
-
 Oryza sativa

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Oryza sativa