Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli and Pichia pastoris | Danio rerio |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0127 | - |
10-formyltetrahydrofolate | 30°C, pH not specified in the publication, recombinant N-terminal domain | Danio rerio | |
0.0156 | - |
10-formyltetrahydrofolate | 30°C, pH not specified in the publication, full-length recombinant enzyme | Danio rerio |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
4 * 100000, calculated | Danio rerio |
400000 | - |
gel fitlration | Danio rerio |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Danio rerio | E3NZ06 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Danio rerio |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.04 | - |
30°C, pH not specified in the publication, recombinant enzyme expressed in Pichia pastoris | Danio rerio |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + NADP+ + H2O | - |
Danio rerio | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
additional information | enzyme does not catalyze the oxidation of 10-formyltetrahydrofolate or propanal | Danio rerio | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 100000, calculated | Danio rerio |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
97 | - |
10-formyltetrahydrofolate | 30°C, pH not specified in the publication, full-length recombinant enzyme | Danio rerio |