Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tetrahydrofolate | very strong product inhibition | Sus scrofa | |
tetrahydropteroylpolyglutamate | inhibition increases with increasing length of polyglutamyl chain | Sus scrofa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
NADP+ | spectrophotometric assay | Sus scrofa | |
0.0006 | - |
NADP+ | fluorimetric assay | Sus scrofa | |
0.0044 | - |
10-formyltetrahydrofolate | racemic, fluorimetric assay | Sus scrofa | |
0.0075 | - |
10-formyltetrahydrofolate | racemic, spectrophotometric assay | Sus scrofa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Sus scrofa | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
92500 | - |
4 * 92500, SDS-PAGE | Sus scrofa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + NADP+ + H2O | Sus scrofa | major disposal route for excess one-carbon units produced in folate-mediated metabolism | tetrahydrofolate + CO2 + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sus scrofa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Sus scrofa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.64 | - |
- |
Sus scrofa |
Storage Stability | Organism |
---|---|
-20°C, dialyzed against 0.01 M potassium phosphate buffer, pH 7.3, 40% glycerol, 0.1 M KCl, several weeks, stable | Sus scrofa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
10-formyltetrahydrofolate + NADP+ + H2O | specific for NADP+ | Sus scrofa | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
10-formyltetrahydrofolate + NADP+ + H2O | major disposal route for excess one-carbon units produced in folate-mediated metabolism | Sus scrofa | tetrahydrofolate + CO2 + NADPH + H+ | - |
? | |
10-formyltetrahydropteroylpolyglutamate + NADP+ + H2O | - |
Sus scrofa | tetrahydropteroylpolyglutamate + CO2 + NADPH + H+ | - |
? | |
additional information | bifunctional enzyme: 10-formyltetrahydrofolate dehydrogenase separated from hydrolase activity, activities are functions of different active sites and take place simultaneously in presence of NADP+ | Sus scrofa | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 92500, SDS-PAGE | Sus scrofa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Sus scrofa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1 | - |
10-formyltetrahydrofolate | - |
Sus scrofa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
10-formyltetrahydrofolate | 10-formyltetrahydrofolate | Sus scrofa | |
NADP+ | NADP+-dependent | Sus scrofa | |
tetrahydrofolate | - |
Sus scrofa |