Literature summary for 1.5.1.54 extracted from

Lu, C.; Liu, Y.; Li, J.; Liu, L.; Du, G.
Engineering of biosynthesis pathway and NADPH supply for improved L-5-methyltetrahydrofolate production by Lactococcus lactis (2021), J. Microbiol. Biotechnol., 31, 154-162 .

Search for more literature for 1.5.1.54

Application

Application	Comment	Organism
synthesis	improvement of the biosynthesis of 5-MTHF by Lactococcus lactis by systematic pathway engineering, including the overexpression of the key rate-limiting enzyme MTHFR, strengthening 5-MTHF biosynthesis and folate supply by coexpression of the drfA, metF, and folE genes, increasing NADPH supply by overexpression of G6PDH, and increasing the proportion of 5-MTHF in various folates by overexpressing 5-formyltetrahydrofolate cyclo-ligase. The 5-MTHF titer finally reaches 300 microg/l with key precursor addition	Lactococcus lactis subsp. lactis

Organism

Organism	UniProt	Comment	Textmining
Lactococcus lactis subsp. lactis	A0A0B8QP67	-	-

Synonyms

Synonyms	Comment	Organism
metF	-	Lactococcus lactis subsp. lactis

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)