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Literature summary for 1.5.1.54 extracted from

  • Sah, S.; Lahry, K.; Talwar, C.; Singh, S.; Varshney, U.
    Monomeric NADH-oxidizing methylenetetrahydrofolate reductases from Mycobacterium smegmatis lack flavin coenzyme (2020), J. Bacteriol., 202, e00709 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Mycolicibacterium smegmatis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0325
-
NADH pH 7.2, 23°C Mycolicibacterium smegmatis
0.0632
-
5,10-methylenetetrahydrofolate pH 7.2, 23°C Mycolicibacterium smegmatis
0.108
-
NADH pH 7.2, 23°C Mycolicibacterium smegmatis
0.145
-
5,10-methylenetetrahydrofolate pH 7.2, 23°C Mycolicibacterium smegmatis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
gel filtration Mycolicibacterium smegmatis
41000
-
gel filtration Mycolicibacterium smegmatis

Organism

Organism UniProt Comment Textmining
Mycolicibacterium smegmatis A0R6M0 isoform MTHFR1
-
Mycolicibacterium smegmatis A0R6S0 isoform MTHFR2
-
Mycolicibacterium smegmatis ATCC 700084 A0R6M0 isoform MTHFR1
-
Mycolicibacterium smegmatis ATCC 700084 A0R6S0 isoform MTHFR2
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5,10-methylenetetrahydrofolate + NADH + H+
-
Mycolicibacterium smegmatis 5-methyltetrahydrofolate + NAD+
-
?
5,10-methylenetetrahydrofolate + NADH + H+
-
Mycolicibacterium smegmatis ATCC 700084 5-methyltetrahydrofolate + NAD+
-
?

Subunits

Subunits Comment Organism
monomer 1 * 34800, SDS-PAGE Mycolicibacterium smegmatis
monomer 1 * 34400, SDS-PAGE Mycolicibacterium smegmatis

Synonyms

Synonyms Comment Organism
MSMEG_6596
-
Mycolicibacterium smegmatis
MSMEG_6649
-
Mycolicibacterium smegmatis
MTHFR2
-
Mycolicibacterium smegmatis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.69
-
5,10-methylenetetrahydrofolate pH 7.2, 23°C Mycolicibacterium smegmatis
1.32
-
NADH pH 7.2, 23°C Mycolicibacterium smegmatis
4.06
-
5,10-methylenetetrahydrofolate pH 7.2, 23°C Mycolicibacterium smegmatis
6.1
-
NADH pH 7.2, 23°C Mycolicibacterium smegmatis

Cofactor

Cofactor Comment Organism Structure
additional information no cofactor: NADPH Mycolicibacterium smegmatis
NADH
-
Mycolicibacterium smegmatis

General Information

General Information Comment Organism
physiological function expression of MTHFR1 rescues an Escherichia coli MTHFR deletion mutant. The Mycolicibacterium smegmatis MTHFR1 mutant strain is partially auxotrophic for methionine and grows only poorly without methionine. The mutant strain is more sensitive to folate pathway inhibitors such as sulfachloropyridazine, 4-aminosalicylic acid, sulfamethoxazole, and trimethoprim Mycolicibacterium smegmatis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.7
-
5,10-methylenetetrahydrofolate pH 7.2, 23°C Mycolicibacterium smegmatis
12.2
-
NADH pH 7.2, 23°C Mycolicibacterium smegmatis
63
-
5,10-methylenetetrahydrofolate pH 7.2, 23°C Mycolicibacterium smegmatis
187
-
NADH pH 7.2, 23°C Mycolicibacterium smegmatis