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Literature summary for 1.5.1.51 extracted from

  • Kobylarz, M.J.; Grigg, J.C.; Takayama, S.J.; Rai, D.K.; Heinrichs, D.E.; Murphy, M.E.
    Synthesis of L-2,3-diaminopropionic acid, a siderophore and antibiotic precursor (2014), Chem. Biol., 21, 379-388.
    View publication on PubMed
Search for more literature for 1.5.1.51

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with NADH and N-(1-amino-1-carboxyl-2-ethyl)-glutamic acid as well as with NAD+ and 2-oxoglutarate, to 1.85 to 2.36 A resolution. NAD+/NADH is bound at full occupancy in a preformed rigid pocket. Each SbnB monomer contains an NAD+/NADH bound at the base of the interdomain channel between the NAD binding and dimerization domains Staphylococcus aureus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38000
-
 2 * 38000, calculated Staphylococcus aureus
71000
-
 and 81000, dynamic and multi-angle light scattering Staphylococcus aureus
81000
-
 and 71000, dynamic and multi-angle light scattering Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-oxoglutarate + L-2,3-diaminopropanoate + NADH + H+ Staphylococcus aureus
-
 N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate + NAD+ + H2O
-
 ?
2-oxoglutarate + L-2,3-diaminopropanoate + NADH + H+ Staphylococcus aureus Newman
-
 N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate + NAD+ + H2O
-
 ?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus A0A0H3K9Y6
-
-
Staphylococcus aureus Newman A0A0H3K9Y6
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + L-2,3-diaminopropanoate + NADH + H+
-
 Staphylococcus aureus N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate + NAD+ + H2O
-
 ?
2-oxoglutarate + L-2,3-diaminopropanoate + NADH + H+
-
 Staphylococcus aureus Newman N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate + NAD+ + H2O
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 38000, calculated Staphylococcus aureus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Staphylococcus aureus

General Information

General Information Comment Organism
physiological function SbnA and SbnB are encoded by the staphyloferrin B biosynthetic gene cluster and are implicated in L-2,3-diaminopropionate biosynthesis. SbnA uses pyridoxal 5'-phosphate and substrates O-phospho-L-serine and L-glutamate to produce a metabolite N-(1-amino-1-carboxyl-2-ethyl)-glutamic acid. SbnB uses NAD+ to oxidatively hydrolyze N-(1-amino-1-carboxyl-2-ethyl)-glutamic acid to yield 2-oxoglutarate and L-2,3-diaminopropionate Staphylococcus aureus