Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Thermoplasma acidophilum |
Crystallization (Comment) | Organism |
---|---|
native form and as a NADP complex, hanging drop vapor diffusion method, at 22°C in 18% PEG 4000, 400 mM NaCl, and 100 mM Tris-HCl (pH 8.0) | Thermoplasma acidophilum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + NADP+ | Thermoplasma acidophilum | - |
5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoplasma acidophilum | Q05213 | - |
- |
Purification (Comment) | Organism |
---|---|
heat treatment, Ni-affinity column chromatography, and Superdex-200 gel filtration | Thermoplasma acidophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + NADP+ | - |
Thermoplasma acidophilum | 5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | x-ray crystallography | Thermoplasma acidophilum |
Synonyms | Comment | Organism |
---|---|---|
5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase | bifunctional enzyme | Thermoplasma acidophilum |
MTHFDC | - |
Thermoplasma acidophilum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Thermoplasma acidophilum |