Literature summary for 1.5.1.5 extracted from

Lee, W.H.; Sung, M.W.; Kim, J.H.; Kim, Y.K.; Han, A.; Hwang, K.Y.
Crystal structure of bifunctional 5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase from Thermoplasma acidophilum (2011), Biochem. Biophys. Res. Commun., 406, 459-463.

Search for more literature for 1.5.1.5

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Thermoplasma acidophilum

Crystallization (Commentary)

Crystallization (Comment)	Organism
native form and as a NADP complex, hanging drop vapor diffusion method, at 22°C in 18% PEG 4000, 400 mM NaCl, and 100 mM Tris-HCl (pH 8.0)	Thermoplasma acidophilum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
5,10-methylenetetrahydrofolate + NADP+	Thermoplasma acidophilum	-	5,10-methenyltetrahydrofolate + NADPH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermoplasma acidophilum	Q05213	-	-

Purification (Commentary)

Purification (Comment)	Organism
heat treatment, Ni-affinity column chromatography, and Superdex-200 gel filtration	Thermoplasma acidophilum

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5,10-methylenetetrahydrofolate + NADP+	-	Thermoplasma acidophilum	5,10-methenyltetrahydrofolate + NADPH + H+	-	?

Subunits

Subunits	Comment	Organism
homodimer	x-ray crystallography	Thermoplasma acidophilum

Synonyms

Synonyms	Comment	Organism
5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase	bifunctional enzyme	Thermoplasma acidophilum
MTHFDC	-	Thermoplasma acidophilum

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Thermoplasma acidophilum

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Release 2023.1 (January 2023)