Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Thermoplasma acidophilum |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion method, in the presence of its cofactor NADP+, at 22°C using polyethylene glycol 4000 as a precipitant | Thermoplasma acidophilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30600 | - |
SDS-PAGE | Thermoplasma acidophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermoplasma acidophilum | - |
strain DSM 1728 | - |
Purification (Comment) | Organism |
---|---|
His-Trap affinity column chromatography | Thermoplasma acidophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,10-methylenetetrahydrofolate + NADP+ | - |
Thermoplasma acidophilum | 5,10-methenyltetrahydrofolate + NADPH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 30600, SDS-PAGE | Thermoplasma acidophilum |
Synonyms | Comment | Organism |
---|---|---|
5,10-methylenetetrahydrofolate dehydrogenase/cyclohydrolase | - |
Thermoplasma acidophilum |
MTHFDC | - |
Thermoplasma acidophilum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | prefers NADP+ as a cofactor, rather than NAD+ | Thermoplasma acidophilum | |
NADP+ | prefers NADP+ as a cofactor, rather than NAD+ | Thermoplasma acidophilum |