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Literature summary for 1.5.1.48 extracted from

  • Li, H.; Zhang, G.; Li, L.; Ou, Y.; Wang, M.; Li, C.; Zheng, G.; Xu, J.
    A novel (R)-imine reductase from Paenibacillus lactis for asymmetric reduction of 3H-indoles (2016), ChemCatChem, 8, 724-727 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
recombinant overexpression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Paenibacillus lactis

Organism

Organism UniProt Comment Textmining
Paenibacillus lactis
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-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) Paenibacillus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,1,2,3-tetramethyl-1H-benzo[e]indol-3-ium iodide + NADPH + H+
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Paenibacillus lactis (-)-1,1,2,3-tetramethyl-2,3-dihydro-1H-benzo[e]indole + NADP+ + hydrogen iodide
-
?
1,2,3,3,5-pentamethyl-3H-indol-1-ium iodide + NADPH + H+
-
Paenibacillus lactis (2S)-1,2,3,3,5-pentamethyl-2,3-dihydro-1H-indole + NADP+ + hydrogen iodide
-
?
1,2,3,3-tetramethyl-3H-indol-1-ium iodide + NADPH + H+
-
Paenibacillus lactis (2S)-1,2,3,3-tetramethyl-2,3-dihydro-1H-indole + NADP+ + hydrogen iodide
-
?
1-ethyl-2,3,3-trimethyl-3H-indol-1-ium iodide + NADPH + H+
-
Paenibacillus lactis (2S)-1-ethyl-2,3,3-trimethyl-2,3-dihydro-1H-indole + NADP+ + hydrogen iodide
-
?
1-methyl-3,4-dihydroisoquinoline + NADPH + H+
-
Paenibacillus lactis (1S)-1-methyl-1,2,3,4-tetrahydroisoquinoline + NADP+
-
?
2,3,3,5-tetramethyl-3H-indole + NADPH + H+
-
Paenibacillus lactis (2R)-2,3,3,5-tetramethyl-2,3-dihydro-1H-indole + NADP+
-
?
2,3,3-trimethyl-1-propyl-3H-indol-1-ium iodide + NADPH + H+
-
Paenibacillus lactis (2S)-2,3,3-trimethyl-1-propyl-2,3-dihydro-1H-indole + NADP+ + hydrogen iodide
-
?
2,3,3-trimethyl-3H-indole + NADPH + H+
-
Paenibacillus lactis (2R)-2,3,3-trimethyl-2,3-dihydro-1H-indole + NADP+
-
?
2,3,3-trimethyl-5-(trifluoromethyl)-3H-indole + NADH + H+
-
Paenibacillus lactis (2R)-2,3,3-trimethyl-5-(trifluoromethyl)-2,3-dihydro-1H-indole + NADP+
-
?
2,3,3-trimethyl-5-nitro-3H-indole + NADPH + H+
-
Paenibacillus lactis (2R)-2,3,3-trimethyl-5-nitro-2,3-dihydro-1H-indole + NADP+
-
?
2,3,3-trimethylindolenine + NADPH + H+ conversion rate of over 81% and enantiomeric excess of also over 96% for the (R)-isomer Paenibacillus lactis (R)-2,3,3-trimethylindoline + NADP+
-
?
2-methyl-1-pyrroline + NADPH + H+
-
Paenibacillus lactis (R)-2-methylpyrrolidine + NADP+
-
?
4,6-difluoro-2,3,3-trimethyl-3H-indole + NADPH + H+
-
Paenibacillus lactis (2R)-4,6-difluoro-2,3,3-trimethyl-2,3-dihydro-1H-indole + NADP+
-
?
5-bromo-2,3,3-trimethyl-3H-indole + NADPH + H+
-
Paenibacillus lactis (2R)-5-bromo-2,3,3-trimethyl-2,3-dihydro-1H-indole + NADP+
-
?
5-chloro-2,3,3-trimethyl-3H-indole + NADPH + H+
-
Paenibacillus lactis (2R)-5-chloro-2,3,3-trimethyl-2,3-dihydro-1H-indole + NADP+
-
?
5-fluoro-1,2,3,3-tetramethyl-3H-indol-1-ium iodide + NADPH + H+
-
Paenibacillus lactis (2S)-5-fluoro-1,2,3,3-tetramethyl-2,3-dihydro-1H-indole + NADP+ + hydrogen iodide
-
?
5-fluoro-2,3,3-trimethyl-3H-indole + NADPH + H+
-
Paenibacillus lactis (2S)-2-phenylpyrrolidine + NADP+
-
?
5-methoxy-2,3,3-trimethyl-3H-indole + NADPH + H+
-
Paenibacillus lactis (2R)-5-methoxy-2,3,3-trimethyl-2,3-dihydro-1H-indole + NADP+
-
?
6-methyl-2,3,4,5-tetrahydropyridine + NADPH + H+
-
Paenibacillus lactis (2S)-2-methylpiperidine + NADP+
-
?
additional information the purified recombinant enzyme PlRIR exhibits relatively high catalytic efficiency towards 2,3,3-trimethylindolenine. A panel of 3H-indoles and 3H-indole iodides are reduced by PlRIR to yield the corresponding products with high enantioselectivity of 66-98% enantiomeric excess. In addition, enzyme PlRIR also possesses good activities toward other types of imines such as pyrroline, tetrahydropyridine, and dihydroisoquinoline, indicating a reasonably broad substrate acceptance Paenibacillus lactis ?
-
?

Synonyms

Synonyms Comment Organism
(R)-imine reductase
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Paenibacillus lactis
PlRIR
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Paenibacillus lactis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Paenibacillus lactis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Paenibacillus lactis

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Paenibacillus lactis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.58
-
2,3,3-trimethylindolenine recombinant enzyme, pH 7.0, 30°C Paenibacillus lactis