Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli as a six-histidine tag | Methylorubrum extorquens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
17000 | - |
2 * 17000, SDS-PAGE | Methylorubrum extorquens |
38500 | - |
gel filtration | Methylorubrum extorquens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydromethanopterin + NAD(P)H + H+ | Methylorubrum extorquens | the enzyme is involved in tetrahydromethanopterin biosynthesis | 5,6,7,8-tetrahydromethanopterin + NAD(P)+ | - |
? | |
7,8-dihydromethanopterin + NAD(P)H + H+ | Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | the enzyme is involved in tetrahydromethanopterin biosynthesis | 5,6,7,8-tetrahydromethanopterin + NAD(P)+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylorubrum extorquens | C5B2R8 | - |
- |
Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | C5B2R8 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Methylorubrum extorquens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.8 | - |
pH 5.3, 30°C | Methylorubrum extorquens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydromethanopterin + NAD(P)H + H+ | the enzyme is involved in tetrahydromethanopterin biosynthesis | Methylorubrum extorquens | 5,6,7,8-tetrahydromethanopterin + NAD(P)+ | - |
? | |
7,8-dihydromethanopterin + NAD(P)H + H+ | dihydrofolate is not a substrate, no activity with reduced methyl viologen | Methylorubrum extorquens | 5,6,7,8-tetrahydromethanopterin + NAD(P)+ | - |
? | |
7,8-dihydromethanopterin + NAD(P)H + H+ | the enzyme is involved in tetrahydromethanopterin biosynthesis | Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | 5,6,7,8-tetrahydromethanopterin + NAD(P)+ | - |
? | |
7,8-dihydromethanopterin + NAD(P)H + H+ | dihydrofolate is not a substrate, no activity with reduced methyl viologen | Methylorubrum extorquens ATCC 14718 / DSM 1338 / JCM 2805 / NCIMB 9133 / AM1 | 5,6,7,8-tetrahydromethanopterin + NAD(P)+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 17000, SDS-PAGE | Methylorubrum extorquens |
Synonyms | Comment | Organism |
---|---|---|
DmrA | - |
Methylorubrum extorquens |
H2MPT reductase | - |
Methylorubrum extorquens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Methylorubrum extorquens |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 35 | 20°C: about 90% of maximal activit, 35°C: about 70% of maximal activity | Methylorubrum extorquens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.3 | - |
- |
Methylorubrum extorquens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.8 | 6.3 | pH 4.8: about 55% of maximal activity, pH 6.3: about 70% of maximal activity | Methylorubrum extorquens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | the specific activity with NADH is 15% of the rate observed with NADPH | Methylorubrum extorquens | |
NADPH | the specific activity with NADH is 15% of the rate observed with NADPH | Methylorubrum extorquens |