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Literature summary for 1.5.1.42 extracted from

  • Kamali, N.; Tavallaie, M.; Bambai, B.; Karkhane, A.A.; Miri, M.
    Site-directed mutagenesis enhances the activity of NADH-FMN oxidoreductase (DszD) activity of Rhodococcus erythropolis (2010), Biotechnol. Lett., 32, 921-927.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Rhodococcus erythropolis

Protein Variants

Protein Variants Comment Organism
T62A the mutant shows 7fold increase in activity compared to the wild type enzyme Rhodococcus erythropolis
T62N the mutant shows 5fold increase in activity compared to the wild type enzyme Rhodococcus erythropolis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22000
-
x * 22000, SDS-PAGE Rhodococcus erythropolis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
FMN + NADH + H+ Rhodococcus erythropolis
-
FMNH2 + NAD+
-
?
FMN + NADH + H+ Rhodococcus erythropolis IGTS8 (ATCC 53968)
-
FMNH2 + NAD+
-
?

Organism

Organism UniProt Comment Textmining
Rhodococcus erythropolis
-
-
-
Rhodococcus erythropolis IGTS8 (ATCC 53968)
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FMN + NADH + H+
-
Rhodococcus erythropolis FMNH2 + NAD+
-
?
FMN + NADH + H+
-
Rhodococcus erythropolis IGTS8 (ATCC 53968) FMNH2 + NAD+
-
?

Subunits

Subunits Comment Organism
? x * 22000, SDS-PAGE Rhodococcus erythropolis

Synonyms

Synonyms Comment Organism
DszD
-
Rhodococcus erythropolis
NADH-FMN oxidoreductase
-
Rhodococcus erythropolis

Cofactor

Cofactor Comment Organism Structure
NADH dependent on Rhodococcus erythropolis