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Literature summary for 1.5.1.36 extracted from

  • Okai, M.; Ohtsuka, J.; Asano, A.; Guo, L.; Miyakawa, T.; Miyazono, K.; Nakamura, A.; Okada, A.; Zheng, H.; Kimura, K.; Nagata, K.; Tanokura, M.
    High pressure refolding, purification, and crystallization of flavin reductase from Sulfolobus tokodaii strain 7 (2012), Protein Expr. Purif., 84, 214-218.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta(DE3) cells Sulfurisphaera tokodaii

Crystallization (Commentary)

Crystallization (Comment) Organism
complexed with FMN, FAD or riboflavin, sitting drop vapor diffusion method, using 1.6 M ammonium sulfate and 4-5% (v/v) isopropanol Sulfurisphaera tokodaii

Organism

Organism UniProt Comment Textmining
Sulfurisphaera tokodaii
-
-
-
Sulfurisphaera tokodaii 7
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-affinity chromatography and heat treatment Sulfurisphaera tokodaii

Renatured (Commentary)

Renatured (Comment) Organism
-
Sulfurisphaera tokodaii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FMN + NADH
-
Sulfurisphaera tokodaii FMNH2 + NAD+
-
?
FMN + NADH
-
Sulfurisphaera tokodaii 7 FMNH2 + NAD+
-
?

Synonyms

Synonyms Comment Organism
HpaC
-
Sulfurisphaera tokodaii

Cofactor

Cofactor Comment Organism Structure
NADH
-
Sulfurisphaera tokodaii
NADPH
-
Sulfurisphaera tokodaii