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Literature summary for 1.5.1.34 extracted from

  • Perez-Reinado, E.; Roldan, M.D.; Castillo, F.; Moreno-Vivian, C.
    The NprA nitroreductase required for 2,4-dinitrophenol reduction in Rhodobacter capsulatus is a dihydropteridine reductase (2008), Environ. Microbiol., 10, 3174-3183.
    View publication on PubMed
Search for more literature for 1.5.1.34

Cloned(Commentary)

Cloned (Comment) Organism
expressed as His-tag fusion protein in Escherichia coli Rhodobacter capsulatus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
25000
-
 x * 25000, SDS-PAGE, weak band at 50000 Da which possibly corresponds to the dimeric form of the enzyme, calculated mass of the His-tagged protein is 24200 Da Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rhodobacter capsulatus in vivo role as dihydropteridine reductase involved in aromatic amino acids metabolism suggested ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rhodobacter capsulatus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein using His-tag Rhodobacter capsulatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6,7-dimethyl-7,8-dihydropterin + NADPH + H+ as the quinonoid forms of pterines are very unstable, the reaction is carried out with a dimethyl analogue in the presence of H2O2 and radish peroxidase to allow the continuous formation of 6,7-dimethyl-7,8-dihydropterine, activity is 25fold higher than the nitroreductase activity 2,4-dinitrophenol Rhodobacter capsulatus 6,7-dimethyl-5,6,7,8-tetrahydropterin + NADP+
-
 ?
additional information in vivo role as dihydropteridine reductase involved in aromatic amino acids metabolism suggested Rhodobacter capsulatus ?
-
 ?
additional information enzyme shows nitroreductase activity with several different substrates such as 2,4-dinitrophenol, 2,4-dinitrobenzoate and nitrofurazone Rhodobacter capsulatus ?
-
 ?

Subunits

Subunits Comment Organism
More x * 25000, SDS-PAGE, weak band at 50000 Da which possibly corresponds to the dimeric form of the enzyme, calculated mass of the His-tagged protein is 24200 Da Rhodobacter capsulatus

Synonyms

Synonyms Comment Organism
NprA nitroreductase enzyme shows nitroreductase activity with a wide range of nitroaromatic compounds, but highest activity is detected with the quinonoid form of 6,7-dimethyl-7,8-dihydropterine as substrate, in vivo role as dihydropteridine reductase involved in aromatic amino acids metabolism suggested Rhodobacter capsulatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Rhodobacter capsulatus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Rhodobacter capsulatus

Cofactor

Cofactor Comment Organism Structure
FMN one molecule bound per enzyme Rhodobacter capsulatus
NADPH
-
 Rhodobacter capsulatus