Crystallization (Comment) | Organism |
---|---|
hanging drop vapour diffusion, polyethylene glycol 4500, various concentrations of Tris phosphate buffer, pH 7.8, 8% methanol and 1 mM beta-mercaptoethanol, 2.5 A resolution | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00015 | - |
quinonoid 7,8-dihydrobiopterin | recombinant enzyme | Homo sapiens | |
0.009 | - |
NADH | recombinant enzyme | Homo sapiens | |
0.018 | - |
quinonoid 2-amino-4-hydroxy-6,7-dimethyl-7,8-dihydropteridine | recombinant enzyme | Homo sapiens | |
0.135 | - |
NADPH | recombinant enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
412 | - |
recombinant enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6,7-dihydropteridine + NADH | - |
Homo sapiens | (6R)-L-erythro-5,6,7,8-tetrahydrobiopterin + NAD+ | - |
? | |
6,7-dimethyl-7,8-dihydropterin + NADPH + H+ | - |
Homo sapiens | 6,7-dimethyl-5,6,7,8-tetrahydropterin + NADP+ | - |
? | |
quinoid dihydrobiopterin + NAD(P)H | - |
Homo sapiens | tetrahydrobiopterin + NAD(P)+ | - |
? | |
quinonoid dihydrobiopterin + NADH + H+ | - |
Homo sapiens | 6R-(1'R,2'R)-5,6,7,8-tetrahydrobiopterin + NAD+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
17 | - |
NADH | recombinant enzyme | Homo sapiens | |
17 | - |
quinoid 7,8-dihydrobiopterin | recombinant enzyme | Homo sapiens | |
20 | - |
NADPH | recombinant enzyme | Homo sapiens | |
162 | - |
quinoid 2-amino-4-hydroxy-6,7-dimethyl-7,8-dihydropteridine | recombinant enzyme | Homo sapiens |