General Stability | Organism |
---|---|
not stable during gel filtration | Crithidia fasciculata |
unstable to (NH4)2SO4 precipitation | Crithidia fasciculata |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
aminopterin | 1 mM, 50% inhibition | Crithidia fasciculata | |
methopterin | 1 mM, 10% inhibition | Crithidia fasciculata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0028 | - |
NADH | - |
Crithidia fasciculata | |
0.0088 | - |
NADPH | - |
Crithidia fasciculata |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
55000 | - |
gel filtration | Crithidia fasciculata |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Crithidia fasciculata | - |
- |
- |
Storage Stability | Organism |
---|---|
-80°C, 40 mM potassium phosphate buffer, pH 6.8, stable for at least a week | Crithidia fasciculata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H | more specific for NADH, 50% activity with NADPH, no activity with biopterin and 7,8-dihydrobiopterin | Crithidia fasciculata | 5,6,7,8-tetrahydrobiopterin + NAD(P)+ | - |
? | |
quinonoid dihydrobiopterin + NADH + H+ | - |
Crithidia fasciculata | 5,6,7,8-tetrahydrobiopterin + NAD+ | - |
r |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | unstable below and above | Crithidia fasciculata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | utilizes both NADH and NADPH | Crithidia fasciculata | |
NADH | enzyme prefers NADH | Crithidia fasciculata | |
NADPH | utilizes both NADH and NADPH | Crithidia fasciculata | |
NADPH | NADH is preferred | Crithidia fasciculata |