KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00085 | - |
NADH | - |
Rattus norvegicus | |
0.00085 | - |
NADH | NADH-specific liver enzyme | Rattus norvegicus | |
0.0034 | - |
quinonoiddihydro-6-methylpterin | - |
Rattus norvegicus | |
0.076 | - |
NADPH | NADH-specific liver enzyme | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
25000 | - |
2 * 25000, NADH-specific liver enzyme, SDS-PAGE | Rattus norvegicus |
49000 | - |
- |
Bos taurus |
49000 | - |
NADH-specific liver enzyme, gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H | Homo sapiens | - |
5,6,7,8-tetrahydrobiopterin + NAD(P)+ | - |
? | |
quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H | Bos taurus | - |
5,6,7,8-tetrahydrobiopterin + NAD(P)+ | - |
? | |
quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H | Rattus norvegicus | essential component of catecholamine biosynthetic metabolism | 5,6,7,8-tetrahydrobiopterin + NAD(P)+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Homo sapiens | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
simple two step procedure i.e. affinity chromatography on Matrex gel blue A and hydrophobic chromatography on phenyl-Sepharose | Homo sapiens |
simple two step procedure i.e. affinity chromatography on Matrex gel blue A and hydrophobic chromatography on phenyl-Sepharose | Rattus norvegicus |
simple two step procedure i.e. affinity chromatography on Matrex gel blue A and hydrophobic chromatography on phenyl-Sepharose | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Homo sapiens | - |
liver | - |
Rattus norvegicus | - |
liver | - |
Bos taurus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20.2 | - |
NADH-specific liver enzyme | Homo sapiens |
24 | - |
NADH-specific liver enzyme | Rattus norvegicus |
26.2 | - |
NADH-specific liver enzyme | Bos taurus |
64.2 | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H | - |
Homo sapiens | 5,6,7,8-tetrahydrobiopterin + NAD(P)+ | - |
? | |
quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H | - |
Bos taurus | 5,6,7,8-tetrahydrobiopterin + NAD(P)+ | - |
? | |
quinonoid 7,8-(6H)-dihydrobiopterin + NAD(P)H | essential component of catecholamine biosynthetic metabolism | Rattus norvegicus | 5,6,7,8-tetrahydrobiopterin + NAD(P)+ | - |
? | |
quinonoiddihydro-6-methylpterin + NADH + H+ | - |
Rattus norvegicus | ? | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Homo sapiens |
dimer | - |
Bos taurus |
dimer | 2 * 25000, NADH-specific liver enzyme, SDS-PAGE | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
NADH-specific liver enzyme | Rattus norvegicus |