Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) GOLD cells | Leishmania donovani |
Crystallization (Comment) | Organism |
---|---|
sitting drop vapor diffusion method, using 0.1 M MES pH 6.5, 10%(v/v) dioxane and 1.6 M ammonium sulfate | Leishmania donovani |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
methotrexate | - |
Leishmania donovani |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
biopterin + 2 NADPH + 2 H+ | Leishmania donovani | - |
5,6,7,8-tetrahydrobiopterin + 2 NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania donovani | Q6QDB5 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap nickel-chelating column chromatography | Leishmania donovani |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
biopterin + 2 NADPH + 2 H+ | - |
Leishmania donovani | 5,6,7,8-tetrahydrobiopterin + 2 NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | x-ray crystallography | Leishmania donovani |
Synonyms | Comment | Organism |
---|---|---|
NADPH-dependent short-chain dehydrogenase/reductase pteridine reductase | - |
Leishmania donovani |
PTR1 | - |
Leishmania donovani |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Leishmania donovani |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000039 | - |
methotrexate | pH and temperature not specified in the publication | Leishmania donovani |