Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-Deaza-5,6,7,8-tetrahydrofolate | - |
Leishmania major | |
additional information | relatively insensitive to methorexate | Leishmania major | |
NADP+ | - |
Leishmania major |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
folate | - |
Leishmania major | |
0.0014 | - |
NADPH | with folate as cosubstrate | Leishmania major |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania major | - |
- |
- |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
5,6,7,8-tetrahydrobiopterin + 2 NADP+ = biopterin + 2 NADPH + 2 H+ | ordered ternary complex mechanism with NADPH binding first and NADP+ dissociating after the reduced pteridine. The enzyme transfers the pro-S hydride of NADPH to carbon 6 on the si face of dihydrofolate | Leishmania major |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Biopterin + NADPH | - |
Leishmania major | 5,6,7,8-Tetrahydrobiopterin + NADP+ | - |
? | |
folate + NADPH | - |
Leishmania major | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Leishmania major |