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Literature summary for 1.5.1.33 extracted from

  • Wang, J.; Leblanc, E.; Chang, C.F.; Papadopoulou, B.; Bray, T.; Witheley, J.M.; Lin, S.X.; Quellette, M.
    Pterin and folate reduction by the Leishmania tarentolae H locus short-chain dehydrogenase/reductase PTR1 (1997), Arch. Biochem. Biophys., 342, 197-202.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Leishmania tarentolae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00351
-
biopterin
-
Leishmania tarentolae
0.0169
-
NADPH
-
Leishmania tarentolae
0.033
-
dihydrobiopterin
-
Leishmania tarentolae
0.085
-
folate
-
Leishmania tarentolae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
2 * 31000, SDS-PAGE Leishmania tarentolae
116800
-
gel filtration Leishmania tarentolae

Organism

Organism UniProt Comment Textmining
Leishmania tarentolae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme Leishmania tarentolae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Biopterin + NADPH
-
Leishmania tarentolae 5,6,7,8-Tetrahydrobiopterin + NADP+
-
?
Dihydrobiopterin + NADPH
-
Leishmania tarentolae ?
-
?
folate + NADPH
-
Leishmania tarentolae 5,6,7,8-tetrahydrofolate + NADP+
-
?

Subunits

Subunits Comment Organism
tetramer 2 * 31000, SDS-PAGE Leishmania tarentolae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
biopterin as substrate Leishmania tarentolae

pH Range

pH Minimum pH Maximum Comment Organism
4.5 7 pH 4.5: about 50% of maximal activity, pH 7.0: about 30% of maximal activity with biopterin Leishmania tarentolae