Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Leishmania tarentolae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00351 | - |
biopterin | - |
Leishmania tarentolae | |
0.0169 | - |
NADPH | - |
Leishmania tarentolae | |
0.033 | - |
dihydrobiopterin | - |
Leishmania tarentolae | |
0.085 | - |
folate | - |
Leishmania tarentolae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
2 * 31000, SDS-PAGE | Leishmania tarentolae |
116800 | - |
gel filtration | Leishmania tarentolae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania tarentolae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Leishmania tarentolae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Biopterin + NADPH | - |
Leishmania tarentolae | 5,6,7,8-Tetrahydrobiopterin + NADP+ | - |
? | |
Dihydrobiopterin + NADPH | - |
Leishmania tarentolae | ? | - |
? | |
folate + NADPH | - |
Leishmania tarentolae | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | 2 * 31000, SDS-PAGE | Leishmania tarentolae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
biopterin as substrate | Leishmania tarentolae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.5 | 7 | pH 4.5: about 50% of maximal activity, pH 7.0: about 30% of maximal activity with biopterin | Leishmania tarentolae |