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Literature summary for 1.5.1.30 extracted from

  • Wang, H.; Lei, B.; Tu, S.C.
    Vibrio harveyi NADPH-FMN oxidoreductase Arg203 as a critical residue for NADPH recognition and binding (2000), Biochemistry, 39, 7813-7819.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Vibrio harveyi

Protein Variants

Protein Variants Comment Organism
R203A decrease of NADPH binding potential, critical role of Arg203 in the specific recognition and binding of NADPH Vibrio harveyi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0058
-
FMN flavin reductase P R203A Vibrio harveyi
0.0069
-
FMN native flavin reductase P Vibrio harveyi
0.021
-
NADPH native flavin reductase P Vibrio harveyi
0.71
-
NADPH flavin reductase P R203A Vibrio harveyi

Organism

Organism UniProt Comment Textmining
Vibrio harveyi
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
FMN + NADPH
-
Vibrio harveyi FMNH2 + NADP+
-
?

Synonyms

Synonyms Comment Organism
NADPH:FMN oxidoreductase
-
Vibrio harveyi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
53.8
-
NADPH flavin reductase P R203A Vibrio harveyi
69.5
-
NADPH native flavin reductase P Vibrio harveyi

Cofactor

Cofactor Comment Organism Structure
FMN
-
Vibrio harveyi
NADPH
-
Vibrio harveyi