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Literature summary for 1.5.1.30 extracted from
- An NADPH:FAD oxidoreductase from the valanimycin producer, Streptomyces viridifaciens. Cloning analysis, and overexpression (1997), J. Biol. Chem., 272, 23303-23311.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Streptomyces viridifaciens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0036 | - |
FAD | - |
Streptomyces viridifaciens |  |
| 0.0084 | - |
FMN | - |
Streptomyces viridifaciens | |
| 0.011 | - |
riboflavin | - |
Streptomyces viridifaciens | |
| 0.032 | - |
NADPH | FAD as electron acceptor | Streptomyces viridifaciens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21270 | - |
calculated from sequence of cloned DNA | Streptomyces viridifaciens |
| 23000 | - |
SDS-PAGE | Streptomyces viridifaciens |
| 36000 | - |
gel filtration | Streptomyces viridifaciens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| riboflavin + NADPH | Streptomyces viridifaciens | - |
reduced riboflavin + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Streptomyces viridifaciens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Streptomyces viridifaciens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 107 | - |
- |
Streptomyces viridifaciens |
| 141 | - |
without N-terminal His-tag | Streptomyces viridifaciens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| FAD + NADPH | - |
Streptomyces viridifaciens | FADH2 + NADP+ | - |
? | |
| FMN + NADPH | - |
Streptomyces viridifaciens | FMNH2 + NADP+ | - |
? | |
| riboflavin + NADPH | - |
Streptomyces viridifaciens | reduced riboflavin + NADP+ | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | - |
Streptomyces viridifaciens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| NADPH:FAD oxidoreductase | - |
Streptomyces viridifaciens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
enzyme assay | Streptomyces viridifaciens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 6.7 | - |
riboflavin | - |
Streptomyces viridifaciens | |
| 45 | - |
FMN | - |
Streptomyces viridifaciens | |
| 51 | - |
FAD | - |
Streptomyces viridifaciens | |
| 62 | - |
NADPH | - |
Streptomyces viridifaciens | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Streptomyces viridifaciens | |
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