Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Haloarcula japonica |
General Stability | Organism |
---|---|
addition of NaCl (0500 mM concentration) induces significant structural formation to the enzyme. Protein stability increases depending on NaCl concentration regardless of structural formation, and HjDHFR P1 achieves the same stability as the Escherichia coli enzyme at 750 mM NaCl | Haloarcula japonica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Haloarcula japonica | AB986558 | - |
- |
Haloarcula japonica TR-1 | AB986558 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Haloarcula japonica |
Synonyms | Comment | Organism |
---|---|---|
folA3 | - |
Haloarcula japonica |
HjDHFR | - |
Haloarcula japonica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
68 | - |
lowering the pH from 8 to 6 induces significant structural formation to the enzyme | Haloarcula japonica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | induces significant structural formation to the enzyme | Haloarcula japonica |