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Literature summary for 1.5.1.3 extracted from
- Cloning and characterization of dihydrofolate reductases from deep-sea bacteria (2010), J. Biochem., 147, 591-599.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli | Escherichia coli |
| expressed in Escherichia coli | Shewanella violacea |
| expressed in Escherichia coli | Photobacterium profundum |
| expressed in Escherichia coli | Moritella yayanosii |
| expressed in Escherichia coli | Moritella japonica |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.001 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Escherichia coli |  |
| 0.0018 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Moritella yayanosii | |
| 0.0019 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Escherichia coli | |
| 0.0019 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Shewanella violacea | |
| 0.0034 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Shewanella violacea | |
| 0.0062 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Photobacterium profundum | |
| 0.0198 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Photobacterium profundum | |
| 0.0584 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Moritella japonica | |
| 0.101 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Moritella yayanosii | |
| 0.238 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Moritella japonica | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Moritella japonica | D1MX72 | - |
- |
| Moritella yayanosii | D1MX71 | - |
- |
| Photobacterium profundum | D1MX70 | - |
- |
| Shewanella violacea | D1MYR1 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using affinity chromatography | Escherichia coli |
| using affinity chromatography | Shewanella violacea |
| using affinity chromatography | Photobacterium profundum |
| using affinity chromatography | Moritella yayanosii |
| using affinity chromatography | Moritella japonica |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
ecDHFR shows a monotonous decrease of its activity with increasing pressure, down to 50% at 250 MPa | Escherichia coli |
| additional information | - |
mjDHFR loses its activity significantly even in the low-pressure region and almost completely above 100 MPa, which may be related to its low substrate-binding ability | Moritella japonica |
| additional information | - |
ppDHFR shows a monotonous decrease of its activity with increasing pressure, down to 20% at 250 MPa | Photobacterium profundum |
| additional information | - |
the activity is invariant below 75 MPa and then gradually decreases with increasing pressure down to 30% at 250 MPa | Moritella yayanosii |
| additional information | - |
the enzymatic activity of svDHFR increases with pressure to 110% at around 75 MPa and then decreases to 60% at 250 MPa | Shewanella violacea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| dihydrofolate + NADPH + H+ | - |
Escherichia coli | tetrahydrofolate + NADP+ | - |
? | |
| dihydrofolate + NADPH + H+ | - |
Shewanella violacea | tetrahydrofolate + NADP+ | - |
? | |
| dihydrofolate + NADPH + H+ | - |
Photobacterium profundum | tetrahydrofolate + NADP+ | - |
? | |
| dihydrofolate + NADPH + H+ | - |
Moritella yayanosii | tetrahydrofolate + NADP+ | - |
? | |
| dihydrofolate + NADPH + H+ | - |
Moritella japonica | tetrahydrofolate + NADP+ | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| dihydrofolate reductase | - |
Escherichia coli |
| dihydrofolate reductase | - |
Shewanella violacea |
| dihydrofolate reductase | - |
Photobacterium profundum |
| dihydrofolate reductase | - |
Moritella yayanosii |
| dihydrofolate reductase | - |
Moritella japonica |
| ecDHFR | - |
Escherichia coli |
| mjDHFR | - |
Moritella japonica |
| myDHFR | - |
Moritella yayanosii |
| ppDHFR | - |
Photobacterium profundum |
| svDHFR | - |
Shewanella violacea |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Escherichia coli |
| 25 | - |
assay at | Shewanella violacea |
| 25 | - |
assay at | Photobacterium profundum |
| 25 | - |
assay at | Moritella yayanosii |
| 25 | - |
assay at | Moritella japonica |
| 25 | - |
optimal temperature | Moritella yayanosii |
| 30 | - |
optimal temperature | Moritella japonica |
| 40 | - |
optimal temperature | Shewanella violacea |
| 53 | - |
optimal temperature | Photobacterium profundum |
| 57 | - |
optimal temperature | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 18.4 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Escherichia coli | |
| 19.2 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Escherichia coli | |
| 80.3 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Moritella yayanosii | |
| 83.8 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Moritella yayanosii | |
| 86.8 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Shewanella violacea | |
| 90 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Shewanella violacea | |
| 109 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Moritella japonica | |
| 144 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Moritella japonica | |
| 150.5 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Photobacterium profundum | |
| 156 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Photobacterium profundum | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
optimal pH | Moritella japonica |
| 7 | - |
assay at | Escherichia coli |
| 7 | - |
assay at | Shewanella violacea |
| 7 | - |
assay at | Photobacterium profundum |
| 7 | - |
assay at | Moritella yayanosii |
| 7 | - |
assay at | Moritella japonica |
| 7 | - |
optimal pH | Shewanella violacea |
| 7 | - |
optimal pH | Photobacterium profundum |
| 7.5 | - |
optimal pH | Moritella yayanosii |
| 8 | - |
optimal pH | Escherichia coli |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 600 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Moritella japonica | |
| 800 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Moritella yayanosii | |
| 1900 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Moritella japonica | |
| 7600 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Photobacterium profundum | |
| 10100 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Escherichia coli | |
| 16700 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Escherichia coli | |
| 25200 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Photobacterium profundum | |
| 25500 | - |
NADPH | pH 7.0, 25°C, under atmospheric pressure | Shewanella violacea | |
| 46600 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Moritella yayanosii | |
| 47400 | - |
dihydrofolate | pH 7.0, 25°C, under atmospheric pressure | Shewanella violacea | |
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