Literature summary for 1.5.1.3 extracted from

Ghosh, P.; Cheng, J.; Chou, T.F.; Jia, Y.; Avdulov, S.; Bitterman, P.B.; Polunovsky, V.A.; Wagner, C.R.
Expression, purification and characterization of recombinant mouse translation initiation factor eIF4E as a dihydrofolate reductase (DHFR) fusion protein (2008), Protein Expr. Purif., 60, 132-139.

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Application

Application	Comment	Organism
synthesis	develoment of a method for bacterial expression of mouse translation factor eIF4E tagged with mutant dihydrofolate reductase. Recombinant eIF4E and DHFR-eIF4E both show to significantly enhance in vitro translation in dose dependent manner by 75% at 0.0005 mM. Increased concentrations of eIF4E and DHFR-eIF4E significantly inhibit translation in a dose dependent manner by a maximum at 0.0022 mM of 60% and 90%, respectively	Mus musculus

Organism

Organism	UniProt	Comment	Textmining
Mus musculus	-	-	-

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Release 2023.1 (January 2023)