Protein Variants | Comment | Organism |
---|---|---|
additional information | pyrimethamine-resistant quadruple mutant 51I/59R/108N/213A, kcat value 0.68 per s, Km value for dihydrofolate, 0.0102 mM, Km value for NADPH, 0.0309 mM | Plasmodium falciparum |
N51I/C59R/S108N | pyrimethamine-resistant mutant, 50% decrease in Km value | Plasmodium falciparum |
N51I/C59R/S108N/I164L | pyrimethamine-resistant mutant, 50% decrease in Km value | Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0207 | - |
7,8-dihydrofolate | mutant N51I/C59R/S108N/I164L , pH 8.0, 25°C | Plasmodium falciparum | |
0.0209 | - |
7,8-dihydrofolate | mutant N51I/C59R/S108N, pH 8.0, 25°C | Plasmodium falciparum | |
0.0232 | - |
NADPH | wild-type, pH 8.0, 25°C | Plasmodium falciparum | |
0.0373 | - |
NADPH | mutant N51I/C59R/S108N/I164L , pH 8.0, 25°C | Plasmodium falciparum | |
0.0379 | - |
NADPH | mutant N51I/C59R/S108N, pH 8.0, 25°C | Plasmodium falciparum | |
0.0444 | - |
7,8-dihydrofolate | wild-type, pH 8.0, 25°C | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH + H+ | - |
Plasmodium falciparum | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.23 | - |
7,8-dihydrofolate | mutant N51I/C59R/S108N, pH 8.0, 25°C | Plasmodium falciparum | |
1.24 | - |
7,8-dihydrofolate | wild-type, pH 8.0, 25°C | Plasmodium falciparum | |
1.42 | - |
7,8-dihydrofolate | mutant N51I/C59R/S108N/I164L , pH 8.0, 25°C | Plasmodium falciparum |