Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of 19 mutants of residue W48. All mutants show very low specific activites | Plasmodium falciparum |
W48Y | 3% of wild-type activity | Plasmodium falciparum |
W48Y/N188Y | 28.9% of wild-type activity | Plasmodium falciparum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
cycloguanil | - |
Plasmodium falciparum | |
additional information | in presence of up to 4 M urea, wild-type retains more than 80% of its catalytic activity. Mutant W48Y loses more than 20% of activity in presence of 1 M urea | Plasmodium falciparum | |
pyrimethamine | - |
Plasmodium falciparum | |
WR99210 | - |
Plasmodium falciparum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0027 | - |
NADPH | wild-type, 25°C, pH 7.0 | Plasmodium falciparum | |
0.00345 | - |
NADPH | mutant W48Y/N188Y, 25°C, pH 7.0 | Plasmodium falciparum | |
0.0045 | - |
NADPH | mutant W48Y, 25°C, pH 7.0 | Plasmodium falciparum | |
0.0051 | - |
7,8-dihydrofolate | wild-type, 25°C, pH 7.0 | Plasmodium falciparum | |
0.0231 | - |
7,8-dihydrofolate | mutant W48Y, 25°C, pH 7.0 | Plasmodium falciparum | |
0.0324 | - |
7,8-dihydrofolate | mutant W48Y/N188Y, 25°C, pH 7.0 | Plasmodium falciparum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.82 | - |
wild-type, 25°C, pH 7.0 | Plasmodium falciparum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH + H+ | - |
Plasmodium falciparum | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.8 | - |
NADPH | mutant W48Y, 25°C, pH 7.0 | Plasmodium falciparum | |
3.3 | - |
NADPH | mutant W48Y/N188Y, 25°C, pH 7.0 | Plasmodium falciparum | |
60.9 | - |
NADPH | wild-type, 25°C, pH 7.0 | Plasmodium falciparum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 6.5 | mutant W48Y, more than 60% of maximum activity | Plasmodium falciparum |
4 | 7.5 | wild-type, more than 60% of maximum activity | Plasmodium falciparum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000044 | - |
WR99210 | wild-type, 25°C, pH 7.0 | Plasmodium falciparum | |
0.00000072 | - |
pyrimethamine | wild-type, 25°C, pH 7.0 | Plasmodium falciparum | |
0.000001 | - |
pyrimethamine | mutant W48Y/N188Y, 25°C, pH 7.0 | Plasmodium falciparum | |
0.000001 | - |
cycloguanil | wild-type, 25°C, pH 7.0 | Plasmodium falciparum | |
0.00000121 | - |
WR99210 | mutant W48Y/N188Y, 25°C, pH 7.0 | Plasmodium falciparum | |
0.00000154 | - |
WR99210 | mutant W48Y, 25°C, pH 7.0 | Plasmodium falciparum | |
0.00000174 | - |
pyrimethamine | mutant W48Y, 25°C, pH 7.0 | Plasmodium falciparum | |
0.00000199 | - |
cycloguanil | mutant W48Y/N188Y, 25°C, pH 7.0 | Plasmodium falciparum | |
0.00000548 | - |
cycloguanil | mutant W48Y, 25°C, pH 7.0 | Plasmodium falciparum |