Crystallization (Comment) | Organism |
---|---|
mutant M1A/M16N/M20L/M42Y/M92F/C85A/C152S, increased structural flexibility and an increased size of the N-(p-aminobenzoyl)-L-glutamate binding cleft | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
C85A/C152S | kinetic properties similar to wild-type | Escherichia coli |
M1A/C85A/C152S | kinetic properties similar to wild-type | Escherichia coli |
M1A/M16F/M20L/M42Y/M92F/C85A/C152S | hyperactive mutant, increase in dissociation rate constant of tetrahydrofolate from the enzyme-NADPH-tetrahydrofolate ternary complex | Escherichia coli |
M1A/M16N/M20L/M42Y/M92F/C85A/C152S | hyperactive mutant, increase in dissociation rate constant of tetrahydrofolate from the enzyme-NADPH-tetrahydrofolate ternary complex | Escherichia coli |
M1A/M16S/M20L/M42Y/M92F/C85A/C152S | hyperactive mutant, increase in dissociation rate constant of tetrahydrofolate from the enzyme-NADPH-tetrahydrofolate ternary complex | Escherichia coli |
M1P/C85A/C152S | kinetic properties similar to wild-type | Escherichia coli |
M1S/C85A/C152S | kinetic properties similar to wild-type | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0012 | - |
7,8-dihydrofolate | mutant C85A/C152S, 15°C, pH 7.0 | Escherichia coli | |
0.0012 | - |
7,8-dihydrofolate | mutant M1P/C85A/C152S, 15°C, pH 7.0 | Escherichia coli | |
0.0012 | - |
7,8-dihydrofolate | wild-type, 15°C, pH 7.0 | Escherichia coli | |
0.0013 | - |
7,8-dihydrofolate | mutant M1A/C85A/C152S, 15°C, pH 7.0 | Escherichia coli | |
0.0025 | - |
7,8-dihydrofolate | mutant M1S/C85A/C152S, 15°C, pH 7.0 | Escherichia coli | |
0.003 | - |
7,8-dihydrofolate | mutant M1A/M16F/M20L/M42Y/M92F/C85A/C152S, 15°C, pH 7.0 | Escherichia coli | |
0.0038 | - |
7,8-dihydrofolate | mutant M1A/M16S/M20L/M42Y/M92F/C85A/C152S, 15°C, pH 7.0 | Escherichia coli | |
0.0083 | - |
7,8-dihydrofolate | mutant M1A/M16N/M20L/M42Y/M92F/C85A/C152S, 15°C, pH 7.0 | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0ABQ4 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH | - |
Escherichia coli | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.6 | - |
7,8-dihydrofolate | mutant M1A/C85A/C152S, 15°C, pH 7.0 | Escherichia coli | |
4.8 | - |
7,8-dihydrofolate | mutant M1P/C85A/C152S, 15°C, pH 7.0 | Escherichia coli | |
4.9 | - |
7,8-dihydrofolate | mutant C85A/C152S, 15°C, pH 7.0 | Escherichia coli | |
5.3 | - |
7,8-dihydrofolate | mutant M1S/C85A/C152S, 15°C, pH 7.0 | Escherichia coli | |
5.3 | - |
7,8-dihydrofolate | wild-type, 15°C, pH 7.0 | Escherichia coli | |
16.2 | - |
7,8-dihydrofolate | mutant M1A/M16S/M20L/M42Y/M92F/C85A/C152S, 15°C, pH 7.0 | Escherichia coli | |
25.8 | - |
7,8-dihydrofolate | mutant M1A/M16F/M20L/M42Y/M92F/C85A/C152S, 15°C, pH 7.0 | Escherichia coli | |
40.5 | - |
7,8-dihydrofolate | mutant M1A/M16N/M20L/M42Y/M92F/C85A/C152S, 15°C, pH 7.0 | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Escherichia coli | |
NADPH | - |
Escherichia coli |