Inhibitors | Comment | Organism | Structure |
---|---|---|---|
folate | - |
Tetradesmus obliquus | |
methotrexate | - |
Tetradesmus obliquus | |
additional information | thymidylate synthase activity inhibitors | Tetradesmus obliquus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
50000 | - |
2 * 50000, thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other, SDS-PAGE | Tetradesmus obliquus |
100000 | - |
thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other, sucrose density gradient centrifugation | Tetradesmus obliquus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tetradesmus obliquus | - |
green algae, thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other | - |
Purification (Comment) | Organism |
---|---|
green algae, thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other | Tetradesmus obliquus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
15.8 | - |
- |
Tetradesmus obliquus |
Storage Stability | Organism |
---|---|
5°C, enzyme after affinity chromatography, half-life 2 days | Tetradesmus obliquus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH | - |
Tetradesmus obliquus | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 50000, thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other, SDS-PAGE | Tetradesmus obliquus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 30 | - |
Tetradesmus obliquus |
25 | - |
assay at | Tetradesmus obliquus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other | Tetradesmus obliquus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 9 | activity negligible below and above | Tetradesmus obliquus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Tetradesmus obliquus |