Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-methyltetrahydrofolate | - |
Leishmania tropica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Leishmania tropica | |
0.0015 | - |
7,8-dihydrofolate | - |
Leishmania tropica | |
0.0027 | - |
NADPH | - |
Leishmania tropica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56400 | - |
2 * 56400, methotrexate-resistant, each subunit is a bifunctional thymidylate synthase-dihydrofolate reductase, SDS-PAGE | Leishmania tropica |
110000 | - |
methotrexate resistant, bifunctional, glycerol density gradient centrifugation | Leishmania tropica |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leishmania tropica | - |
methotrexate resistant bifunctional enzyme: thymidylate synthase-dihydrofolate reductase | - |
Purification (Comment) | Organism |
---|---|
- |
Leishmania tropica |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Leishmania tropica |
Storage Stability | Organism |
---|---|
-20°C, 40% glycerol, pH 7.4, 5 mM dithiothreitol, 1 mM EDTA, 1 month | Leishmania tropica |
4°C, 40-50% loss of activity after 1 month | Leishmania tropica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,8-dihydrofolate + NADPH | - |
Leishmania tropica | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 56400, methotrexate-resistant, each subunit is a bifunctional thymidylate synthase-dihydrofolate reductase, SDS-PAGE | Leishmania tropica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Leishmania tropica |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.15 | - |
5-methyltetrahydrofolate | - |
Leishmania tropica |