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Literature summary for 1.5.1.3 extracted from

  • Poe, M.; Breeze, A.S.; Wu, J.K.; Short, C.R.; Hoogsteen, K.
    Dihydrofolate reductase from trimethoprim-resistant Escherichia coli MB 3746 and MB 3747. Purification, amino acid composition, and some kinetic properties (1979), J. Biol. Chem., 254, 1799-1805.
    View publication on PubMed

General Stability

General Stability Organism
7,8-dihydrofolate and folate protect against ethoxyformic anhydride modification Escherichia coli
7,8-dihydrofolate protects against heat inactivation Escherichia coli
7,8-dihydrofolate protects against inactivation Escherichia coli
NADPH, protects against ethoxyformic anhydride modification Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
2,4-diaminopyrimidine
-
Escherichia coli
aminopterin
-
Escherichia coli
folate
-
Escherichia coli
methotrexate
-
Escherichia coli
trimethoprim
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17300 18300 trimethoprim-resistant Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
strains: MB3746, MB 3747 of K12
-
Escherichia coli
-
trimethoprim-resistant strain
-

Purification (Commentary)

Purification (Comment) Organism
strain MB 3746, MB 3747 Escherichia coli
trimethoprim-resistant Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
below, strain 3747 Escherichia coli
7.6
-
strain 1428 Escherichia coli
8
-
strain 3746 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Escherichia coli

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.000001
-
trimethoprim strains MB 1428 and MB 3746 Escherichia coli
0.000011
-
trimethoprim strain MB 3747 Escherichia coli