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Literature summary for 1.5.1.3 extracted from
- Purification and properties of Escherichia coli dihydrofolate reductase (1975), Biochemistry, 14, 5267-5273.
General Stability
| General Stability | Organism |
|---|---|
| freezing and thawing, stable to repeated freezing and thawing | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ba2+ | - |
Escherichia coli |  |
| Ca2+ | - |
Escherichia coli | |
| Cs+ | - |
Escherichia coli | |
| Diaminobutane | 12 mM | Escherichia coli | |
| Diaminopentane | 12 mM | Escherichia coli | |
| ethylenediamine | 12 mM | Escherichia coli | |
| K+ | - |
Escherichia coli | |
| Li+ | - |
Escherichia coli | |
| Mg2+ | - |
Escherichia coli | |
| additional information | inhibitory effect increased with the radius of the cations | Escherichia coli | |
| Na+ | - |
Escherichia coli | |
| Rb+ | - |
Escherichia coli | |
| spermidine | 12 mM | Escherichia coli | |
| spermine | 12 mM | Escherichia coli | |
| Urea | 3 M, 15% inhibition | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Escherichia coli |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 17300 | 18300 | trimethoprim-resistant, amino acid composition, sedimentation velocity | Escherichia coli |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
trimethoprim-resistant strain | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| trimethoprim-resistant | Escherichia coli |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 19.1 | - |
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C for several weeks | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
trimethoprim resistant, 2 optima: pH 4.5 and pH 7.0 | Escherichia coli |
| 4.5 | - |
folic acid reduction | Escherichia coli |
| 7 | - |
trimethoprim resistant, 2 optima: pH 4.5 and pH 7.0 | Escherichia coli |
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Release 2023.1 (January 2023)
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