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Literature summary for 1.5.1.3 extracted from
- Purification and properties of dihydrofolate reductase from Crithidia fasciculata (1981), Agric. Biol. Chem., 45, 113-120.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| 2-mercaptoethanol, 4 mM, stabilizes purified preparation, inclusion in purification steps causes loss of activity | Crithidia fasciculata |
| glycerol, 30%, stabilization of purified preparation | Crithidia fasciculata |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| aminopterin | - |
Crithidia fasciculata |  |
| chaotrophes | e.g. guanidine-HCl; formamide; urea | Crithidia fasciculata | |
| K+ | - |
Crithidia fasciculata | |
| methotrexate | - |
Crithidia fasciculata | |
| N-ethylmaleimide | - |
Crithidia fasciculata | |
| p-chloromercuribenzoate | - |
Crithidia fasciculata | |
| pyrimethamine | - |
Crithidia fasciculata | |
| trimethoprim | - |
Crithidia fasciculata | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0011 | - |
7,8-dihydrofolate | - |
Crithidia fasciculata | |
| 0.0027 | - |
NADPH | - |
Crithidia fasciculata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
2 * 58000, isoform IIa, SDS-PAGE | Crithidia fasciculata |
| 110000 | - |
isoform IIa, gel filtration | Crithidia fasciculata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Crithidia fasciculata | - |
three different forms I, IIa and IIb | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isoform IIa | Crithidia fasciculata |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| denaturation with 4 M urea, no renaturation by removing urea by dialysis | Crithidia fasciculata |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 6.8 | - |
- |
Crithidia fasciculata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydrofolate + NADPH | reverse reaction at only one tenth of forward reaction | Crithidia fasciculata | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 58000, isoform IIa, SDS-PAGE | Crithidia fasciculata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Crithidia fasciculata |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADH | not | Crithidia fasciculata | |
| NADPH | - |
Crithidia fasciculata | |
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Release 2023.1 (January 2023)
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