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Literature summary for 1.5.1.3 extracted from

  • Poe, M.; Greenfield, N.J.; Hirshfield, J.M.; Williams, M.N.; Hoogsteen, K.
    Dihydrofolate reductase. Purification and characterization of the enzyme from an amethopterin-resistant mutant of Escherichia coli (1972), Biochemistry, 11, 1023-1030.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
K+
-
Escherichia coli
methotrexate
-
Escherichia coli
Na+
-
Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00044
-
7,8-dihydrofolate
-
Escherichia coli
0.00645
-
NADPH
-
Escherichia coli
0.32
-
NADH
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
17300 18300 methotrexate-resistant mutant, equilibrium sedimentation Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-
Escherichia coli
-
methotrexate-resistant mutant
-
Escherichia coli MB 1428 / B / ATCC 11303
-
-
-

Purification (Commentary)

Purification (Comment) Organism
methotrexate-resistant mutant: large-scale Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
36
-
methotrexate resistant, 23°C Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7,8-dihydrofolate + NADH + H+
-
Escherichia coli 5,6,7,8-tetrahydrofolate + NAD+
-
r
7,8-dihydrofolate + NADH + H+
-
Escherichia coli MB 1428 / B / ATCC 11303 5,6,7,8-tetrahydrofolate + NAD+
-
r
folate + NADPH no activity Escherichia coli 5,6,7,8-tetrahydrofolate + NADP+
-
?
folate + NADPH no activity Escherichia coli MB 1428 / B / ATCC 11303 5,6,7,8-tetrahydrofolate + NADP+
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
10
-
NADPH per binding site Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
methotrexate-resistant cells, broad maximum Escherichia coli

pH Range

pH Minimum pH Maximum Comment Organism
4.2 8.8 half-maximal activities at pH 4.2 and pH 8.8, acetate buffer, Tris buffer Escherichia coli

Cofactor

Cofactor Comment Organism Structure
NADH NADPH oxidized 5.1-times more rapidly than NADH Escherichia coli
NADPH NADPH oxidized 5.1-times more rapidly than NADH Escherichia coli