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Literature summary for 1.5.1.3 extracted from
- Dihydrofolate reductase (1976), Methods Cancer Res., 13, 199-225.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| methotrexate | - |
Bos taurus |  |
| methotrexate | - |
Cricetulus sp. | |
| methotrexate | - |
Enterococcus faecalis | |
| methotrexate | - |
Escherichia coli | |
| methotrexate | - |
Gallus gallus | |
| methotrexate | - |
Lacticaseibacillus casei | |
| methotrexate | - |
Mammalia | |
| methotrexate | - |
Mus musculus | |
| methotrexate | - |
Sus scrofa | |
| methotrexate | - |
Tequatrovirus T4 | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Mammalia | |
| additional information | - |
additional information | - |
Enterococcus faecalis | |
| additional information | - |
additional information | - |
Tequatrovirus T4 | |
| additional information | - |
additional information | overview | Gallus gallus | |
| additional information | - |
additional information | overview | Mus musculus | |
| additional information | - |
additional information | overview | Escherichia coli | |
| additional information | - |
additional information | overview | Sus scrofa | |
| additional information | - |
additional information | overview | Bos taurus | |
| additional information | - |
additional information | overview | Lacticaseibacillus casei | |
| additional information | - |
additional information | overview | Enterococcus faecium | |
| additional information | - |
additional information | overview | Cricetulus sp. |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| additional information | - |
overview | Gallus gallus |
| additional information | - |
overview | Mammalia |
| additional information | - |
overview | Mus musculus |
| additional information | - |
overview | Sus scrofa |
| additional information | - |
overview | Bos taurus |
| additional information | - |
overview | Cricetulus sp. |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydrofolate + NADPH | Mammalia | - |
5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| 7,8-dihydrofolate + NADPH | Gallus gallus | maintainance of adequate levels of fully reduced folate in metabolism of proliferating cells | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| 7,8-dihydrofolate + NADPH | Sus scrofa | maintainance of adequate levels of fully reduced folate in metabolism of proliferating cells | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| 7,8-dihydrofolate + NADPH | Bos taurus | maintainance of adequate levels of fully reduced folate in metabolism of proliferating cells | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| 7,8-dihydrofolate + NADPH | Cricetulus sp. | maintainance of adequate levels of fully reduced folate in metabolism of proliferating cells | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
| Cricetulus sp. | - |
baby hamster kidney cells | - |
| Enterococcus faecalis | - |
- |
- |
| Enterococcus faecium | - |
- |
- |
| Escherichia coli | - |
amethopterin-resistant | - |
| Gallus gallus | - |
- |
- |
| Lacticaseibacillus casei | - |
amethopterin-resistant | - |
| Mammalia | - |
- |
- |
| Mus musculus | - |
L1210 lymphoma cells, amethopterin-resistant | - |
| Sus scrofa | - |
- |
- |
| Tequatrovirus T4 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| overview | Mus musculus |
| overview | Sus scrofa |
| overview | Bos taurus |
| overview | Enterococcus faecalis |
| overview | Enterococcus faecium |
| overview | Tequatrovirus T4 |
| overview | Cricetulus sp. |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| kidney | - |
Cricetulus sp. | - |
| liver | - |
Gallus gallus | - |
| liver | - |
Mus musculus | - |
| liver | - |
Sus scrofa | - |
| liver | - |
Bos taurus | - |
| thymus | - |
Sus scrofa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
| additional information | - |
- |
Lacticaseibacillus casei |
| additional information | - |
- |
Tequatrovirus T4 |
| additional information | - |
enzyme assay | Gallus gallus |
| additional information | - |
enzyme assay | Sus scrofa |
| additional information | - |
enzyme assay | Bos taurus |
| additional information | - |
enzyme assay | Cricetulus sp. |
| additional information | - |
overview | Gallus gallus |
| additional information | - |
overview | Mus musculus |
| additional information | - |
overview | Sus scrofa |
| additional information | - |
overview | Bos taurus |
| additional information | - |
overview | Cricetulus sp. |
| 11.6 | - |
amethopterin-resistant cells L1210/S | Mus musculus |
| 23 | - |
- |
Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 7,8-dihydrofolate + NADPH | - |
Gallus gallus | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| 7,8-dihydrofolate + NADPH | - |
Mammalia | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| 7,8-dihydrofolate + NADPH | - |
Mus musculus | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| 7,8-dihydrofolate + NADPH | - |
Sus scrofa | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| 7,8-dihydrofolate + NADPH | - |
Bos taurus | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| 7,8-dihydrofolate + NADPH | - |
Bos taurus | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
| 7,8-dihydrofolate + NADPH | - |
Cricetulus sp. | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| 7,8-dihydrofolate + NADPH | equilibrium strongly favors tetrahydrofolate production | Mammalia | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| 7,8-dihydrofolate + NADPH | equilibrium strongly favors tetrahydrofolate production | Mammalia | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
| 7,8-dihydrofolate + NADPH | equilibrium strongly favors tetrahydrofolate production | Escherichia coli | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| 7,8-dihydrofolate + NADPH | equilibrium strongly favors tetrahydrofolate production | Escherichia coli | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
| 7,8-dihydrofolate + NADPH | equilibrium strongly favors tetrahydrofolate production | Enterococcus faecalis | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
| 7,8-dihydrofolate + NADPH | equilibrium strongly favors tetrahydrofolate production | Lacticaseibacillus casei | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| 7,8-dihydrofolate + NADPH | equilibrium strongly favors tetrahydrofolate production | Lacticaseibacillus casei | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
| 7,8-dihydrofolate + NADPH | equilibrium strongly favors tetrahydrofolate production | Enterococcus faecium | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| 7,8-dihydrofolate + NADPH | equilibrium strongly favors tetrahydrofolate production | Enterococcus faecium | 5,6,7,8-tetrahydrofolate + NADP+ | - |
r | |
| 7,8-dihydrofolate + NADPH | maintainance of adequate levels of fully reduced folate in metabolism of proliferating cells | Gallus gallus | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| 7,8-dihydrofolate + NADPH | maintainance of adequate levels of fully reduced folate in metabolism of proliferating cells | Sus scrofa | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| 7,8-dihydrofolate + NADPH | maintainance of adequate levels of fully reduced folate in metabolism of proliferating cells | Bos taurus | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| 7,8-dihydrofolate + NADPH | maintainance of adequate levels of fully reduced folate in metabolism of proliferating cells | Cricetulus sp. | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| folate + NADPH | - |
Gallus gallus | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| folate + NADPH | - |
Mus musculus | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| folate + NADPH | - |
Sus scrofa | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| folate + NADPH | - |
Bos taurus | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| folate + NADPH | - |
Cricetulus sp. | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| folate + NADPH | at a slow rate, reaction not catalyzed by all dihydrofolate reductases | Mammalia | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? | |
| additional information | - |
Escherichia coli | ? | - |
? | |
| additional information | equilibrium strongly favors tetrahydrofolate production | Gallus gallus | ? | - |
? | |
| additional information | equilibrium strongly favors tetrahydrofolate production | Sus scrofa | ? | - |
? | |
| additional information | equilibrium strongly favors tetrahydrofolate production | Bos taurus | ? | - |
? | |
| additional information | equilibrium strongly favors tetrahydrofolate production | Cricetulus sp. | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
overview | Gallus gallus |
| additional information | - |
overview | Mus musculus |
| additional information | - |
overview | Sus scrofa |
| additional information | - |
overview | Bos taurus |
| additional information | - |
overview | Cricetulus sp. |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Escherichia coli |
| additional information | - |
overview | Gallus gallus |
| additional information | - |
overview | Mus musculus |
| additional information | - |
overview | Sus scrofa |
| additional information | - |
overview | Bos taurus |
| additional information | - |
overview | Cricetulus sp. |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Enterococcus faecium |
| additional information | - |
- |
Cricetulus sp. |
| additional information | - |
overview | Mammalia |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Gallus gallus | |
| NADPH | - |
Mammalia | |
| NADPH | - |
Mus musculus | |
| NADPH | - |
Escherichia coli | |
| NADPH | - |
Sus scrofa | |
| NADPH | - |
Bos taurus | |
| NADPH | - |
Enterococcus faecalis | |
| NADPH | - |
Lacticaseibacillus casei | |
| NADPH | - |
Enterococcus faecium | |
| NADPH | - |
Tequatrovirus T4 | |
| NADPH | - |
Cricetulus sp. | |
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