Literature summary for 1.5.1.27 extracted from

  • De-Eknamkul, W.; Zenk, M.H.
    Purification and properties of 1,2-dehydroreticuline reductase from Papaver somniferum seedlings (1992), Phytochemistry, 31, 813-821.
No PubMed abstract available

General Stability

General Stability Organism
stablilized by NADPH, 1 mM Papaver somniferum

Inhibitors

Inhibitors Comment Organism Structure
6-O-methyllaudanosoline
-
Papaver somniferum
codeine
-
Papaver somniferum
morphine
-
Papaver somniferum
oripavine
-
Papaver somniferum
salutaridine
-
Papaver somniferum
(S)-Coclaurine
-
Papaver somniferum
(S)-N-methylcoclaurine
-
Papaver somniferum
(S)-norreticuline
-
Papaver somniferum
(S)-reticuline
-
Papaver somniferum
thebaine
-
Papaver somniferum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.01
-
1,2-dehydroreticuline
-
Papaver somniferum
0.007
-
NADPH
-
Papaver somniferum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Papaver somniferum
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
gel filtration Papaver somniferum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1,2-Dehydroreticuline + NADPH Papaver somniferum the enzyme catalyzes the provision of (R)-reticuline for the formation of morphinian alkaloids that possess also (R)-configuration at the chiral centre ?
-
?

Organism

Organism UniProt Comment Textmining
no activity in Argemone hunnemannii
-
-
-
no activity in Dicentra spectabilis
-
-
-
no activity in Fumaria macrosepta
-
-
-
no activity in Papaver oreophilum
-
-
-
no activity in Papaver persicum
-
-
-
Papaver bracteatum
-
-
-
Papaver somniferum
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
Papaver somniferum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Papaver somniferum

Storage Stability

Storage Stability Organism
4°C, 1 mM NADPH, 50% loss of activity after 20 days Papaver somniferum
-20°C, 1 mM NADPH, 50% loss of activity after 30 days Papaver bracteatum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,2-dehydroreticuline + NADPH + H+
-
Papaver somniferum (R)-reticuline + NADP+
-
?
1,2-dehydroreticuline + NADPH + H+
-
Papaver bracteatum (R)-reticuline + NADP+
-
?
1,2-Dehydroreticuline + NADPH the enzyme catalyzes the provision of (R)-reticuline for the formation of morphinian alkaloids that possess also (R)-configuration at the chiral centre Papaver somniferum ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 30000, SDS-PAGE Papaver somniferum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
Papaver somniferum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
-
Papaver somniferum

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Papaver somniferum