BRENDA - Enzyme Database
show all sequences of 1.5.1.25

Insights into enzyme catalysis and thyroid hormone regulation of cerebral ketimine reductase/mu-crystallin under physiological conditions

Hallen, A.; Cooper, A.J.; Jamie, J.F.; Karuso, P.; Neurochem. Res. 40, 1252-1266 (2015)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
3,3',5'-L-triiodothyronine
competitive inhibition
Homo sapiens
3,5,3'-L-triiodothyronine
competitive inhibition
Homo sapiens
3,5,3'-triiodothyronine
-
Bos taurus
3,5,3'-triiodothyronine
-
Mus musculus
3,5-diiodo-L-tyrosine
low competitive inhibition
Homo sapiens
3,5-diiodothyronine
competitive inhibition
Homo sapiens
4,5-dibromopyrrole-2-carboxylate
-
Bos taurus
4,5-dibromopyrrole-2-carboxylate
-
Homo sapiens
4,5-dibromopyrrole-2-carboxylate
-
Mus musculus
DELTA1-piperideine 2-carboxylate
substrate inhibition
Homo sapiens
L-thyroxine
competitive inhibition
Homo sapiens
L-tyrosine
competitive inhibition
Homo sapiens
additional information
in silico docking of substrates and inhibitors using ketimine reductase/CRYM cyrstal structure, PDB ID 4BVA, overview
Homo sapiens
picolinate
-
Bos taurus
picolinate
; competitive inhibition, picolinate is a much poorer inhibitor than pyrrole-2-carboxylate because it does not possess a ring -NH and relies on a relatively weak ring interaction
Homo sapiens
picolinate
-
Mus musculus
pyrrole-2-carboxylate
-
Bos taurus
pyrrole-2-carboxylate
; competitive inhibition, pyrrole-2-carboxylate is an effective inhibitor of ketimine reductase/CRYM mainly as a result of the -NH hydrogen bonding to an active site residue
Homo sapiens
pyrrole-2-carboxylate
-
Mus musculus
S-(2-aminoethyl)-L-cysteine ketimine
substrate inhibition
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics
Homo sapiens
0.013
-
DELTA1-piperideine 2-carboxylate
pH 7.2, 37°C
Homo sapiens
0.021
-
DELTA2-thiazoline-2-carboxylate
pH 7.2, 37°C
Homo sapiens
0.028
-
S-(2-aminoethyl)-L-cysteine ketimine
pH 7.2, 37°C
Homo sapiens
0.045
-
DELTA1-pyrrolidine 2-carboxylate
pH 7.2, 37°C
Homo sapiens
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Bos taurus
5829
-
cytosol
-
Homo sapiens
5829
-
cytosol
-
Mus musculus
5829
-
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
DELTA1-piperideine 2-carboxylate + NADPH + H+
Mus musculus
-
L-pipecolate + NADP+
-
-
?
DELTA1-piperideine 2-carboxylate + NADPH + H+
Homo sapiens
-
L-pipecolate + NADP+
-
-
?
DELTA1-piperideine 2-carboxylate + NADPH + H+
Bos taurus
-
L-pipecolate + NADP+
-
-
?
DELTA1-pyrrolidine 2-carboxylate + NADPH + H+
Mus musculus
-
L-proline + NADP+
-
-
?
DELTA1-pyrrolidine 2-carboxylate + NADPH + H+
Homo sapiens
-
L-proline + NADP+
-
-
?
DELTA1-pyrrolidine 2-carboxylate + NADPH + H+
Bos taurus
-
L-proline + NADP+
-
-
?
DELTA2-thiazoline-2-carboxylate + NADPH + H+
Mus musculus
-
? + NADP+
-
-
?
DELTA2-thiazoline-2-carboxylate + NADPH + H+
Homo sapiens
-
? + NADP+
-
-
?
DELTA2-thiazoline-2-carboxylate + NADPH + H+
Bos taurus
-
? + NADP+
-
-
?
additional information
Homo sapiens
the non-sulfur substrates exist in equilibrium with open chain forms at low acidic pH. At neutral pH, they exist predominantly as the enzymatically favorable cyclic ketimine form (in which the ring double bond is in the C=N form), while sulfur-containing cyclic ketimine substrates exist predominantly as the enzymatically unfavorable enamine form (in which the ring double bond is in the C=C form) at neutral pH
?
-
-
-
additional information
Bos taurus
the non-sulfur substrates exist in equilibrium with open chain forms at low acidic pH. At neutral pH, they exist predominantly as the enzymatically favorable cyclic ketimine form (in which the ring double bond is in the C=N form), while sulfur-containing cyclic ketimine substrates exist predominantly as the enzymatically unfavorable enamine form (in which the ring double bond is in the C=C form) at neutral pH
?
-
-
-
additional information
Mus musculus
the non-sulfur substrates exist in equilibrium with openchain forms at low acidic pH. At neutral pH, they exist predominantly as the enzymatically favorable cyclic ketimine form (in which the ring double bond is in the C=N form), while sulfur-containing cyclic ketimine substrates exist predominantly as the enzymatically unfavorable enamine form (in which the ring double bond is in the C=C form) at neutral pH
?
-
-
-
S-(2-aminoethyl)-L-cysteine ketimine + NADPH + H+
Mus musculus
-
1,4-thiomorpholine-3-carboxylate + NADP+
-
-
?
S-(2-aminoethyl)-L-cysteine ketimine + NADPH + H+
Homo sapiens
-
1,4-thiomorpholine-3-carboxylate + NADP+
-
-
?
S-(2-aminoethyl)-L-cysteine ketimine + NADPH + H+
Bos taurus
-
1,4-thiomorpholine-3-carboxylate + NADP+
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
-
-
-
Homo sapiens
-
-
-
Mus musculus
-
-
-
Reaction
Reaction
Commentary
Organism
thiomorpholine 3-carboxylate + NAD(P)+ = 3,4-dehydro-thiomorpholine-3-carboxylate + NAD(P)H + H+
in silico docking of various ligands into the active site of the X-ray structure of the enzyme suggests an unusual catalytic mechanism involving an arginine residue as a proton donor
Bos taurus
thiomorpholine 3-carboxylate + NAD(P)+ = 3,4-dehydro-thiomorpholine-3-carboxylate + NAD(P)H + H+
in silico docking of various ligands into the active site of the X-ray structure of the enzyme suggests an unusual catalytic mechanism involving an arginine residue as a proton donor, proposed mechanism for the reaction catalyzed by ketimine reductase/CRYM, overview
Homo sapiens
thiomorpholine 3-carboxylate + NAD(P)+ = 3,4-dehydro-thiomorpholine-3-carboxylate + NAD(P)H + H+
in silico docking of various ligands into the active site of the X-ray structure of the enzyme suggests an unusual catalytic mechanism involving an arginine residue as a proton donor
Mus musculus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
-
Bos taurus
-
brain
-
Homo sapiens
-
brain
-
Mus musculus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
DELTA1-piperideine 2-carboxylate + NADPH + H+
-
743354
Mus musculus
L-pipecolate + NADP+
-
-
-
?
DELTA1-piperideine 2-carboxylate + NADPH + H+
-
743354
Homo sapiens
L-pipecolate + NADP+
-
-
-
?
DELTA1-piperideine 2-carboxylate + NADPH + H+
-
743354
Bos taurus
L-pipecolate + NADP+
-
-
-
?
DELTA1-pyrrolidine 2-carboxylate + NADPH + H+
-
743354
Mus musculus
L-proline + NADP+
-
-
-
?
DELTA1-pyrrolidine 2-carboxylate + NADPH + H+
-
743354
Homo sapiens
L-proline + NADP+
-
-
-
?
DELTA1-pyrrolidine 2-carboxylate + NADPH + H+
-
743354
Bos taurus
L-proline + NADP+
-
-
-
?
DELTA2-thiazoline-2-carboxylate + NADPH + H+
-
743354
Mus musculus
? + NADP+
-
-
-
?
DELTA2-thiazoline-2-carboxylate + NADPH + H+
-
743354
Homo sapiens
? + NADP+
-
-
-
?
DELTA2-thiazoline-2-carboxylate + NADPH + H+
-
743354
Bos taurus
? + NADP+
-
-
-
?
additional information
the non-sulfur substrates exist in equilibrium with open chain forms at low acidic pH. At neutral pH, they exist predominantly as the enzymatically favorable cyclic ketimine form (in which the ring double bond is in the C=N form), while sulfur-containing cyclic ketimine substrates exist predominantly as the enzymatically unfavorable enamine form (in which the ring double bond is in the C=C form) at neutral pH
743354
Homo sapiens
?
-
-
-
-
additional information
the non-sulfur substrates exist in equilibrium with open chain forms at low acidic pH. At neutral pH, they exist predominantly as the enzymatically favorable cyclic ketimine form (in which the ring double bond is in the C=N form), while sulfur-containing cyclic ketimine substrates exist predominantly as the enzymatically unfavorable enamine form (in which the ring double bond is in the C=C form) at neutral pH
743354
Bos taurus
?
-
-
-
-
additional information
the non-sulfur substrates exist in equilibrium with openchain forms at low acidic pH. At neutral pH, they exist predominantly as the enzymatically favorable cyclic ketimine form (in which the ring double bond is in the C=N form), while sulfur-containing cyclic ketimine substrates exist predominantly as the enzymatically unfavorable enamine form (in which the ring double bond is in the C=C form) at neutral pH
743354
Mus musculus
?
-
-
-
-
additional information
in silico docking of substrates and inhibitors using ketimine reductase/CRYM cyrstal structure, PDB ID 4BVA, overview
743354
Homo sapiens
?
-
-
-
-
S-(2-aminoethyl)-L-cysteine ketimine + NADPH + H+
-
743354
Mus musculus
1,4-thiomorpholine-3-carboxylate + NADP+
-
-
-
?
S-(2-aminoethyl)-L-cysteine ketimine + NADPH + H+
-
743354
Homo sapiens
1,4-thiomorpholine-3-carboxylate + NADP+
-
-
-
?
S-(2-aminoethyl)-L-cysteine ketimine + NADPH + H+
-
743354
Bos taurus
1,4-thiomorpholine-3-carboxylate + NADP+
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Homo sapiens
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.2
-
S-(2-aminoethyl)-L-cysteine ketimine
pH 7.2, 37°C
Homo sapiens
1.7
-
DELTA2-thiazoline-2-carboxylate
pH 7.2, 37°C
Homo sapiens
4.4
-
DELTA1-piperideine 2-carboxylate
pH 7.2, 37°C
Homo sapiens
6.6
-
DELTA1-pyrrolidine 2-carboxylate
pH 7.2, 37°C
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
substrate reduction reaction
Bos taurus
7.2
-
substrate reduction reaction
Homo sapiens
7.2
-
reduction reaction of S-(2-aminoethyl)-L-cysteine ketimine
Mus musculus
Cofactor
Cofactor
Commentary
Organism
Structure
NADPH
-
Bos taurus
NADPH
-
Homo sapiens
NADPH
-
Mus musculus
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.000032
-
3,5-diiodothyronine
pH 7.2, 37°C
Homo sapiens
0.000035
-
3,3',5'-L-triiodothyronine
pH 7.2, 37°C
Homo sapiens
0.038
-
4,5-dibromopyrrole-2-carboxylate
pH 7.2, 37°C
Homo sapiens
0.313
-
3,5-diiodo-L-tyrosine
pH 7.2, 37°C
Homo sapiens
1.7
-
S-(2-aminoethyl)-L-cysteine ketimine
pH 7.2, 37°C
Homo sapiens
1.8
-
DELTA1-piperideine 2-carboxylate
pH 7.2, 37°C
Homo sapiens
IC50 Value
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.0027
-
pH 7.2, 37°C
Homo sapiens
pyrrole-2-carboxylate
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADPH
-
Bos taurus
NADPH
-
Homo sapiens
NADPH
-
Mus musculus
IC50 Value (protein specific)
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
0.0027
-
pH 7.2, 37°C
Homo sapiens
pyrrole-2-carboxylate
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3,3',5'-L-triiodothyronine
competitive inhibition
Homo sapiens
3,5,3'-L-triiodothyronine
competitive inhibition
Homo sapiens
3,5,3'-triiodothyronine
-
Bos taurus
3,5,3'-triiodothyronine
-
Mus musculus
3,5-diiodo-L-tyrosine
low competitive inhibition
Homo sapiens
3,5-diiodothyronine
competitive inhibition
Homo sapiens
4,5-dibromopyrrole-2-carboxylate
-
Bos taurus
4,5-dibromopyrrole-2-carboxylate
-
Homo sapiens
4,5-dibromopyrrole-2-carboxylate
-
Mus musculus
DELTA1-piperideine 2-carboxylate
substrate inhibition
Homo sapiens
L-thyroxine
competitive inhibition
Homo sapiens
L-tyrosine
competitive inhibition
Homo sapiens
additional information
in silico docking of substrates and inhibitors using ketimine reductase/CRYM cyrstal structure, PDB ID 4BVA, overview
Homo sapiens
picolinate
-
Bos taurus
picolinate
; competitive inhibition, picolinate is a much poorer inhibitor than pyrrole-2-carboxylate because it does not possess a ring -NH and relies on a relatively weak ring interaction
Homo sapiens
picolinate
-
Mus musculus
pyrrole-2-carboxylate
-
Bos taurus
pyrrole-2-carboxylate
; competitive inhibition, pyrrole-2-carboxylate is an effective inhibitor of ketimine reductase/CRYM mainly as a result of the -NH hydrogen bonding to an active site residue
Homo sapiens
pyrrole-2-carboxylate
-
Mus musculus
S-(2-aminoethyl)-L-cysteine ketimine
substrate inhibition
Homo sapiens
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.000032
-
3,5-diiodothyronine
pH 7.2, 37°C
Homo sapiens
0.000035
-
3,3',5'-L-triiodothyronine
pH 7.2, 37°C
Homo sapiens
0.038
-
4,5-dibromopyrrole-2-carboxylate
pH 7.2, 37°C
Homo sapiens
0.313
-
3,5-diiodo-L-tyrosine
pH 7.2, 37°C
Homo sapiens
1.7
-
S-(2-aminoethyl)-L-cysteine ketimine
pH 7.2, 37°C
Homo sapiens
1.8
-
DELTA1-piperideine 2-carboxylate
pH 7.2, 37°C
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
Michaelis-Menten kinetics
Homo sapiens
0.013
-
DELTA1-piperideine 2-carboxylate
pH 7.2, 37°C
Homo sapiens
0.021
-
DELTA2-thiazoline-2-carboxylate
pH 7.2, 37°C
Homo sapiens
0.028
-
S-(2-aminoethyl)-L-cysteine ketimine
pH 7.2, 37°C
Homo sapiens
0.045
-
DELTA1-pyrrolidine 2-carboxylate
pH 7.2, 37°C
Homo sapiens
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
cytosol
-
Bos taurus
5829
-
cytosol
-
Homo sapiens
5829
-
cytosol
-
Mus musculus
5829
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
DELTA1-piperideine 2-carboxylate + NADPH + H+
Mus musculus
-
L-pipecolate + NADP+
-
-
?
DELTA1-piperideine 2-carboxylate + NADPH + H+
Homo sapiens
-
L-pipecolate + NADP+
-
-
?
DELTA1-piperideine 2-carboxylate + NADPH + H+
Bos taurus
-
L-pipecolate + NADP+
-
-
?
DELTA1-pyrrolidine 2-carboxylate + NADPH + H+
Mus musculus
-
L-proline + NADP+
-
-
?
DELTA1-pyrrolidine 2-carboxylate + NADPH + H+
Homo sapiens
-
L-proline + NADP+
-
-
?
DELTA1-pyrrolidine 2-carboxylate + NADPH + H+
Bos taurus
-
L-proline + NADP+
-
-
?
DELTA2-thiazoline-2-carboxylate + NADPH + H+
Mus musculus
-
? + NADP+
-
-
?
DELTA2-thiazoline-2-carboxylate + NADPH + H+
Homo sapiens
-
? + NADP+
-
-
?
DELTA2-thiazoline-2-carboxylate + NADPH + H+
Bos taurus
-
? + NADP+
-
-
?
additional information
Homo sapiens
the non-sulfur substrates exist in equilibrium with open chain forms at low acidic pH. At neutral pH, they exist predominantly as the enzymatically favorable cyclic ketimine form (in which the ring double bond is in the C=N form), while sulfur-containing cyclic ketimine substrates exist predominantly as the enzymatically unfavorable enamine form (in which the ring double bond is in the C=C form) at neutral pH
?
-
-
-
additional information
Bos taurus
the non-sulfur substrates exist in equilibrium with open chain forms at low acidic pH. At neutral pH, they exist predominantly as the enzymatically favorable cyclic ketimine form (in which the ring double bond is in the C=N form), while sulfur-containing cyclic ketimine substrates exist predominantly as the enzymatically unfavorable enamine form (in which the ring double bond is in the C=C form) at neutral pH
?
-
-
-
additional information
Mus musculus
the non-sulfur substrates exist in equilibrium with openchain forms at low acidic pH. At neutral pH, they exist predominantly as the enzymatically favorable cyclic ketimine form (in which the ring double bond is in the C=N form), while sulfur-containing cyclic ketimine substrates exist predominantly as the enzymatically unfavorable enamine form (in which the ring double bond is in the C=C form) at neutral pH
?
-
-
-
S-(2-aminoethyl)-L-cysteine ketimine + NADPH + H+
Mus musculus
-
1,4-thiomorpholine-3-carboxylate + NADP+
-
-
?
S-(2-aminoethyl)-L-cysteine ketimine + NADPH + H+
Homo sapiens
-
1,4-thiomorpholine-3-carboxylate + NADP+
-
-
?
S-(2-aminoethyl)-L-cysteine ketimine + NADPH + H+
Bos taurus
-
1,4-thiomorpholine-3-carboxylate + NADP+
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
-
Bos taurus
-
brain
-
Homo sapiens
-
brain
-
Mus musculus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
DELTA1-piperideine 2-carboxylate + NADPH + H+
-
743354
Mus musculus
L-pipecolate + NADP+
-
-
-
?
DELTA1-piperideine 2-carboxylate + NADPH + H+
-
743354
Homo sapiens
L-pipecolate + NADP+
-
-
-
?
DELTA1-piperideine 2-carboxylate + NADPH + H+
-
743354
Bos taurus
L-pipecolate + NADP+
-
-
-
?
DELTA1-pyrrolidine 2-carboxylate + NADPH + H+
-
743354
Mus musculus
L-proline + NADP+
-
-
-
?
DELTA1-pyrrolidine 2-carboxylate + NADPH + H+
-
743354
Homo sapiens
L-proline + NADP+
-
-
-
?
DELTA1-pyrrolidine 2-carboxylate + NADPH + H+
-
743354
Bos taurus
L-proline + NADP+
-
-
-
?
DELTA2-thiazoline-2-carboxylate + NADPH + H+
-
743354
Mus musculus
? + NADP+
-
-
-
?
DELTA2-thiazoline-2-carboxylate + NADPH + H+
-
743354
Homo sapiens
? + NADP+
-
-
-
?
DELTA2-thiazoline-2-carboxylate + NADPH + H+
-
743354
Bos taurus
? + NADP+
-
-
-
?
additional information
the non-sulfur substrates exist in equilibrium with open chain forms at low acidic pH. At neutral pH, they exist predominantly as the enzymatically favorable cyclic ketimine form (in which the ring double bond is in the C=N form), while sulfur-containing cyclic ketimine substrates exist predominantly as the enzymatically unfavorable enamine form (in which the ring double bond is in the C=C form) at neutral pH
743354
Homo sapiens
?
-
-
-
-
additional information
the non-sulfur substrates exist in equilibrium with open chain forms at low acidic pH. At neutral pH, they exist predominantly as the enzymatically favorable cyclic ketimine form (in which the ring double bond is in the C=N form), while sulfur-containing cyclic ketimine substrates exist predominantly as the enzymatically unfavorable enamine form (in which the ring double bond is in the C=C form) at neutral pH
743354
Bos taurus
?
-
-
-
-
additional information
the non-sulfur substrates exist in equilibrium with openchain forms at low acidic pH. At neutral pH, they exist predominantly as the enzymatically favorable cyclic ketimine form (in which the ring double bond is in the C=N form), while sulfur-containing cyclic ketimine substrates exist predominantly as the enzymatically unfavorable enamine form (in which the ring double bond is in the C=C form) at neutral pH
743354
Mus musculus
?
-
-
-
-
additional information
in silico docking of substrates and inhibitors using ketimine reductase/CRYM cyrstal structure, PDB ID 4BVA, overview
743354
Homo sapiens
?
-
-
-
-
S-(2-aminoethyl)-L-cysteine ketimine + NADPH + H+
-
743354
Mus musculus
1,4-thiomorpholine-3-carboxylate + NADP+
-
-
-
?
S-(2-aminoethyl)-L-cysteine ketimine + NADPH + H+
-
743354
Homo sapiens
1,4-thiomorpholine-3-carboxylate + NADP+
-
-
-
?
S-(2-aminoethyl)-L-cysteine ketimine + NADPH + H+
-
743354
Bos taurus
1,4-thiomorpholine-3-carboxylate + NADP+
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Homo sapiens
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.2
-
S-(2-aminoethyl)-L-cysteine ketimine
pH 7.2, 37°C
Homo sapiens
1.7
-
DELTA2-thiazoline-2-carboxylate
pH 7.2, 37°C
Homo sapiens
4.4
-
DELTA1-piperideine 2-carboxylate
pH 7.2, 37°C
Homo sapiens
6.6
-
DELTA1-pyrrolidine 2-carboxylate
pH 7.2, 37°C
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.2
-
substrate reduction reaction
Bos taurus
7.2
-
substrate reduction reaction
Homo sapiens
7.2
-
reduction reaction of S-(2-aminoethyl)-L-cysteine ketimine
Mus musculus
General Information
General Information
Commentary
Organism
metabolism
lysine is catabolized in mammalian tissues by two main pathways: the saccharopine pathway and the pipecolate pathway. The pipecolate pathway is the main route for lysine catabolism in the adult brain, whereas the saccharopine pathway predominates in extracerebral tissues. Iimportance of the pipecolate pathway in brain metabolism. Lysine/ornithine catabolism and interconnected pathways in mammalian tissues, and metabolic pathways involving sulfur-containing cyclic ketimines, overview
Bos taurus
metabolism
lysine is catabolized in mammalian tissues by two main pathways: the saccharopine pathway and the pipecolate pathway. The pipecolate pathway is the main route for lysine catabolism in the adult brain, whereas the saccharopine pathway predominates in extracerebral tissues. Importance of the pipecolate pathway in brain metabolism. Lysine/ornithine catabolism and interconnected pathways in mammalian tissues, and metabolic pathways involving sulfur-containing cyclic ketimines, overview
Homo sapiens
metabolism
lysine is catabolized in mammalian tissues by two main pathways: the saccharopine pathway and the pipecolate pathway. The pipecolate pathway is the main route for lysine catabolism in the adult brain, whereas the saccharopine pathway predominates in extracerebral tissues. Importance of the pipecolate pathway in brain metabolism. Lysine/ornithine catabolism and interconnected pathways in mammalian tissues, and metabolic pathways involving sulfur-containing cyclic ketimines, overview
Mus musculus
additional information
in silico docking of various substrates and small inhibitors into the active site of the X-ray structures of mouse ketimine reductase/CRYM in order to better understand the enzyme catalytic mechanism
Mus musculus
General Information (protein specific)
General Information
Commentary
Organism
metabolism
lysine is catabolized in mammalian tissues by two main pathways: the saccharopine pathway and the pipecolate pathway. The pipecolate pathway is the main route for lysine catabolism in the adult brain, whereas the saccharopine pathway predominates in extracerebral tissues. Iimportance of the pipecolate pathway in brain metabolism. Lysine/ornithine catabolism and interconnected pathways in mammalian tissues, and metabolic pathways involving sulfur-containing cyclic ketimines, overview
Bos taurus
metabolism
lysine is catabolized in mammalian tissues by two main pathways: the saccharopine pathway and the pipecolate pathway. The pipecolate pathway is the main route for lysine catabolism in the adult brain, whereas the saccharopine pathway predominates in extracerebral tissues. Importance of the pipecolate pathway in brain metabolism. Lysine/ornithine catabolism and interconnected pathways in mammalian tissues, and metabolic pathways involving sulfur-containing cyclic ketimines, overview
Homo sapiens
metabolism
lysine is catabolized in mammalian tissues by two main pathways: the saccharopine pathway and the pipecolate pathway. The pipecolate pathway is the main route for lysine catabolism in the adult brain, whereas the saccharopine pathway predominates in extracerebral tissues. Importance of the pipecolate pathway in brain metabolism. Lysine/ornithine catabolism and interconnected pathways in mammalian tissues, and metabolic pathways involving sulfur-containing cyclic ketimines, overview
Mus musculus
additional information
in silico docking of various substrates and small inhibitors into the active site of the X-ray structures of mouse ketimine reductase/CRYM in order to better understand the enzyme catalytic mechanism
Mus musculus
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
7
-
S-(2-aminoethyl)-L-cysteine ketimine
pH 7.2, temperature 37°C
Homo sapiens
80
-
DELTA2-thiazoline-2-carboxylate
pH 7.2, 37°C
Homo sapiens
148
-
DELTA1-pyrrolidine 2-carboxylate
pH 7.2, 37°C
Homo sapiens
339
-
DELTA1-piperideine 2-carboxylate
pH 7.2, 37°C
Homo sapiens
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
7
-
S-(2-aminoethyl)-L-cysteine ketimine
pH 7.2, temperature 37°C
Homo sapiens
80
-
DELTA2-thiazoline-2-carboxylate
pH 7.2, 37°C
Homo sapiens
148
-
DELTA1-pyrrolidine 2-carboxylate
pH 7.2, 37°C
Homo sapiens
339
-
DELTA1-piperideine 2-carboxylate
pH 7.2, 37°C
Homo sapiens
Other publictions for EC 1.5.1.25
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741584
Hallen
Ketimine reductase/CRYM catal ...
Homo sapiens
Amino Acids
47
2457-2461
2015
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
7
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
7
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
743354
Hallen
Insights into enzyme catalysi ...
Bos taurus, Homo sapiens, Mus musculus
Neurochem. Res.
40
1252-1266
2015
-
-
-
-
-
-
20
5
3
-
-
15
-
3
-
-
-
3
-
3
-
-
16
-
1
-
-
4
3
-
-
3
6
-
1
-
-
-
3
-
-
-
1
20
6
5
3
-
-
15
-
-
-
-
-
3
-
-
16
-
1
-
-
4
3
-
-
-
-
4
4
-
4
4
741580
Hallen
Lysine metabolism in mammalia ...
Bos taurus, Homo sapiens, Macropus giganteus, Mus musculus, Rattus norvegicus, Sus scrofa
Amino Acids
45
1249-1272
2013
-
-
-
-
-
-
6
-
6
-
-
9
-
6
-
-
4
-
-
16
1
-
9
-
-
-
-
-
1
-
-
6
1
-
-
-
-
-
6
-
-
-
-
6
1
-
6
-
-
9
-
-
-
4
-
16
1
-
9
-
-
-
-
-
1
-
-
-
2
14
14
2
-
-
440358
Nardini
Purification and characterizat ...
Sus scrofa
Eur. J. Biochem.
173
689-694
1988
-
-
-
-
-
1
1
4
-
-
2
-
-
3
-
-
1
1
-
1
1
-
8
-
-
-
-
-
3
-
-
2
-
-
-
-
-
-
2
-
-
1
-
1
-
4
-
-
2
-
-
-
-
1
-
1
1
-
8
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
440359
Nardini
Bovine brain ketimine reductas ...
Bos taurus
Biochim. Biophys. Acta
957
286-292
1988
-
-
-
-
-
-
-
6
1
-
2
-
-
1
-
-
1
1
-
1
1
1
8
1
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
6
1
-
2
-
-
-
-
1
-
1
1
1
8
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-