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Literature summary for 1.5.1.24 extracted from

  • Ruvinov, S.B.; Thompson, J.; Sackett, D.L.; Ginsburg, A.
    Tetrameric N5-(L-1-carboxyethyl)-L-ornithine synthase: Guanidine-HCl-induced unfolding and a low temperature requirement for refolding (1999), Arch. Biochem. Biophys., 371, 115-123.
    View publication on PubMed

Application

Application Comment Organism
nutrition industrially important organism used widely as a starter in the dairy industry Lactococcus lactis

General Stability

General Stability Organism
enzymatic activity of CEOS is lost at guanidine-HCl concentrations from 0.5-1.2 M Lactococcus lactis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34200
-
4 * 34200, sedimentation equilibrium, 20°C, 3.5 M guanidine-HCl Lactococcus lactis
34700
-
4 * 34700, sedimentation equilibrium, 20°C, 3.0 M guanidine-HCl Lactococcus lactis
34900
-
4 * 34900, sedimentation equilibrium, 20°C Lactococcus lactis
35300
-
4 * 35300 Lactococcus lactis
35320
-
deduced from amino acid sequence Lactococcus lactis
38600
-
4 * 38600, sedimentation equilibrium, 20°C Lactococcus lactis
127500
-
sedimentation equilibrium at 20°C, 3.0 M guanidine-HCl Lactococcus lactis
135900
-
sedimentation equilibrium at 20°C, 3.0 M guanidine-HCl Lactococcus lactis
136900
-
sedimentation equilibrium at 20°C Lactococcus lactis
138900
-
sedimentation equilibrium at 20°C, 3.5 M guanidine-HCl Lactococcus lactis
141300
-
gel filtration, analytical ultracentrifugation, sedimentation equilibrium Lactococcus lactis
145700
-
sedimentation equilibrium at 4°C Lactococcus lactis
146700
-
sedimentation equilibrium at 20°C, 3.5 M guanidine-HCl Lactococcus lactis

Organism

Organism UniProt Comment Textmining
Lactococcus lactis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme purified from the overproducing strain Escherichia coli CEO571 Lactococcus lactis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-ornithine + pyruvate + NADPH pyruvic acid serves as an effective keto substrate for the condensation reaction Lactococcus lactis N5-(L-1-carboxyethyl)-L-ornithine + NADP+ + H2O
-
ir
lysine + pyruvate + NADPH
-
Lactococcus lactis N7-(1-carboxyethyl)lysine + NADP+ + H2O
-
ir

Subunits

Subunits Comment Organism
tetramer 4 * 34900, sedimentation equilibrium, 20°C Lactococcus lactis
tetramer 4 * 35300 Lactococcus lactis
tetramer 4 * 34200, sedimentation equilibrium, 20°C, 3.5 M guanidine-HCl Lactococcus lactis
tetramer 4 * 38600, sedimentation equilibrium, 20°C Lactococcus lactis
tetramer 4 * 34700, sedimentation equilibrium, 20°C, 3.0 M guanidine-HCl Lactococcus lactis

Synonyms

Synonyms Comment Organism
CEOS
-
Lactococcus lactis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
heating CEOS in absence of guanidine-HCl produces irreversible denaturation and loss in activity Lactococcus lactis

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Lactococcus lactis