BRENDA - Enzyme Database show
show all sequences of 1.5.1.23

Tauropine dehydrogenase from the sandworm Arabella iricolor (polychaeta: errantia): purification and characterization

Kanno, N.; Sato, M.; Nagahisa, E.; Sato, Y.; Comp. Biochem. Physiol. B 114, 409-416 (1996)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
2-oxobutanoate
slight substrate inhibition
Arabella iricolor
oxaloacetate
slight substrate inhibition
Arabella iricolor
pyruvate
substrate inhibition
Arabella iricolor
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.036
-
NADH
-
Arabella iricolor
0.051
-
NAD+
-
Arabella iricolor
0.34
-
pyruvate
-
Arabella iricolor
3.3
-
2-oxobutanoate
-
Arabella iricolor
4.8
-
tauropine
-
Arabella iricolor
4.9
-
oxaloacetate
-
Arabella iricolor
35.7
-
taurine
-
Arabella iricolor
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43500
-
1 * 43500, SDS-PAGE
Arabella iricolor
43700
-
gel filtration
Arabella iricolor
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Arabella iricolor
the enzyme is a dominant pyruvate reductase in this organism
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Arabella iricolor
-
-
-
Purification (Commentary)
Commentary
Organism
2 enzyme forms: TaDH-1 and TaDH-2
Arabella iricolor
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
878.3
-
-
Arabella iricolor
Storage Stability
Storage Stability
Organism
4C, 2 mM KH2PO4-NaOH, pH 7.2, 1 mM EDTA, 10 mM 2-mercaptoethanol, stable for at least 3 weeks
Arabella iricolor
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Aminomethane sulfonic acid + pyruvate + NADH
at 1.8% of the activity with taurine
13440
Arabella iricolor
N2-(1-Carboxyethyl)aminomethanesulfonic acid + NAD+ + H2O
-
-
-
-
Homotaurine + pyruvate + NADH
at 2.4% of the activity with taurine
13440
Arabella iricolor
?
-
-
-
-
Hypotaurine + pyruvate + NADH
at 40% of the activity with taurine
13440
Arabella iricolor
N2-(1-Carboxyethyl)aminoethanesulfinic acid + NAD+ + H2O
-
-
-
-
additional information
the enzyme is a dominant pyruvate reductase in this organism
13440
Arabella iricolor
?
-
-
-
-
Taurine + 2-oxobutanoate + NADH
-
13440
Arabella iricolor
?
-
-
-
-
Taurine + 2-oxohexanoate + NADH
at 4.8% of the activity with pyruvate
13440
Arabella iricolor
?
-
-
-
-
Taurine + 2-oxopentanoate + NADH
at 7.8% of the activity with pyruvate
13440
Arabella iricolor
?
-
-
-
-
Taurine + 3-hydroxypyruvate + NADH
at 21.2% of the activity with pyruvate
13440
Arabella iricolor
?
-
-
-
-
Taurine + glyoxylate + NADH
at 10.9% of the activity with pyruvate
13440
Arabella iricolor
?
-
-
-
-
taurine + oxaloacetate + NADH
-
13440
Arabella iricolor
?
-
-
-
-
Taurine + pyruvate + NADH
-
13440
Arabella iricolor
Tauropine + NAD+ + H2O
-
13440
Arabella iricolor
-
Subunits
Subunits
Commentary
Organism
monomer
1 * 43500, SDS-PAGE
Arabella iricolor
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.6
7.3
tauropine formation, enzyme form TaDH-1
Arabella iricolor
8.5
-
tauropine oxidation
Arabella iricolor
pH Range
pH Minimum
pH Maximum
Commentary
Organism
5.5
7.9
50% of maximal activity at pH 5.5 and at pH 7.9, tauropine formation, enzyme form TaDH-1
Arabella iricolor
6.8
8.5
50% of maximal activity at pH 6.8, optimum at pH 8.5, tauropine oxidation, enzyme form TaDH-1
Arabella iricolor
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Arabella iricolor
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Arabella iricolor
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-oxobutanoate
slight substrate inhibition
Arabella iricolor
oxaloacetate
slight substrate inhibition
Arabella iricolor
pyruvate
substrate inhibition
Arabella iricolor
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.036
-
NADH
-
Arabella iricolor
0.051
-
NAD+
-
Arabella iricolor
0.34
-
pyruvate
-
Arabella iricolor
3.3
-
2-oxobutanoate
-
Arabella iricolor
4.8
-
tauropine
-
Arabella iricolor
4.9
-
oxaloacetate
-
Arabella iricolor
35.7
-
taurine
-
Arabella iricolor
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
43500
-
1 * 43500, SDS-PAGE
Arabella iricolor
43700
-
gel filtration
Arabella iricolor
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Arabella iricolor
the enzyme is a dominant pyruvate reductase in this organism
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
2 enzyme forms: TaDH-1 and TaDH-2
Arabella iricolor
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
878.3
-
-
Arabella iricolor
Storage Stability (protein specific)
Storage Stability
Organism
4C, 2 mM KH2PO4-NaOH, pH 7.2, 1 mM EDTA, 10 mM 2-mercaptoethanol, stable for at least 3 weeks
Arabella iricolor
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Aminomethane sulfonic acid + pyruvate + NADH
at 1.8% of the activity with taurine
13440
Arabella iricolor
N2-(1-Carboxyethyl)aminomethanesulfonic acid + NAD+ + H2O
-
-
-
-
Homotaurine + pyruvate + NADH
at 2.4% of the activity with taurine
13440
Arabella iricolor
?
-
-
-
-
Hypotaurine + pyruvate + NADH
at 40% of the activity with taurine
13440
Arabella iricolor
N2-(1-Carboxyethyl)aminoethanesulfinic acid + NAD+ + H2O
-
-
-
-
additional information
the enzyme is a dominant pyruvate reductase in this organism
13440
Arabella iricolor
?
-
-
-
-
Taurine + 2-oxobutanoate + NADH
-
13440
Arabella iricolor
?
-
-
-
-
Taurine + 2-oxohexanoate + NADH
at 4.8% of the activity with pyruvate
13440
Arabella iricolor
?
-
-
-
-
Taurine + 2-oxopentanoate + NADH
at 7.8% of the activity with pyruvate
13440
Arabella iricolor
?
-
-
-
-
Taurine + 3-hydroxypyruvate + NADH
at 21.2% of the activity with pyruvate
13440
Arabella iricolor
?
-
-
-
-
Taurine + glyoxylate + NADH
at 10.9% of the activity with pyruvate
13440
Arabella iricolor
?
-
-
-
-
taurine + oxaloacetate + NADH
-
13440
Arabella iricolor
?
-
-
-
-
Taurine + pyruvate + NADH
-
13440
Arabella iricolor
Tauropine + NAD+ + H2O
-
13440
Arabella iricolor
-
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 43500, SDS-PAGE
Arabella iricolor
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.6
7.3
tauropine formation, enzyme form TaDH-1
Arabella iricolor
8.5
-
tauropine oxidation
Arabella iricolor
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
5.5
7.9
50% of maximal activity at pH 5.5 and at pH 7.9, tauropine formation, enzyme form TaDH-1
Arabella iricolor
6.8
8.5
50% of maximal activity at pH 6.8, optimum at pH 8.5, tauropine oxidation, enzyme form TaDH-1
Arabella iricolor
Other publictions for EC 1.5.1.23
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742358
Di
Protein changes in abalone fo ...
Haliotis diversicolor
Ecol. Evol.
6
3645-3657
2016
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1
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3
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1
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2
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1
2
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1
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2
2
-
-
-
741868
Lagana
-
Anaerobiosis and metabolic pl ...
Pinna nobilis
Biochem. Syst. Ecol.
56
138-143
2014
-
-
-
-
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-
1
-
-
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-
-
1
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5
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1
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1
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2
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2
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1
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5
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1
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1
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2
2
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743766
Di
Genetic changes in muscle pro ...
Haliotis discus hannai
Proteomics
13
845-859
2013
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3
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1
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1
-
2
2
-
-
-
688792
Plese
-
Cloning and expression of a ta ...
Suberites domuncula
Mar. Biol.
153
1219-1232
2008
-
-
1
-
-
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1
-
-
-
1
3
-
1
-
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1
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1
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4
-
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2
-
2
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1
2
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1
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1
3
-
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1
-
1
-
-
4
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
655275
Kan-no
The amino acid sequence of tau ...
Arabella iricolor
Comp. Biochem. Physiol. B
140
475-485
2005
-
-
1
-
-
-
-
-
-
-
3
1
-
3
-
1
-
1
-
-
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-
1
2
-
-
-
-
-
-
-
2
-
2
-
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-
1
2
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-
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3
1
-
-
1
-
-
-
-
-
1
2
-
-
-
-
-
-
-
2
-
-
-
-
-
-
673151
Kan-no
-
Tauropine dehydrogenase from t ...
Halichondria japonica
Comp. Biochem. Physiol. B
141B
331-339
2005
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
1
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-
-
-
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1
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1
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1
-
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-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13439
Kan-no
Tauropine dehydrogenase from t ...
Patiria pectinifera
Comp. Biochem. Physiol. B
121
323-332
1998
-
-
-
-
-
-
1
6
-
-
2
-
-
5
-
-
1
-
-
3
1
-
6
1
-
-
-
-
2
2
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
6
-
-
2
-
-
-
-
1
-
3
1
-
6
1
-
-
-
-
2
2
-
-
-
-
-
-
-
-
13437
Kanno
-
Purification and characterizat ...
Halichondria japonica
Fish. Sci.
63
414-420
1997
-
-
-
-
-
1
2
5
-
-
2
-
-
1
-
-
1
-
-
-
1
-
10
1
-
-
-
-
2
2
-
1
-
-
-
-
-
-
1
-
-
1
-
2
-
5
-
-
2
-
-
-
-
1
-
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1
-
10
1
-
-
-
-
2
2
-
-
-
-
-
-
-
-
13440
Kanno
Tauropine dehydrogenase from t ...
Arabella iricolor
Comp. Biochem. Physiol. B
114
409-416
1996
-
-
-
-
-
-
3
7
-
-
2
1
-
2
-
-
1
-
-
-
1
1
11
1
-
-
-
-
2
2
-
1
-
-
-
-
-
-
1
-
-
-
-
3
-
7
-
-
2
1
-
-
-
1
-
-
1
1
11
1
-
-
-
-
2
2
-
-
-
-
-
-
-
-
13291
Hammen
-
Opine oxidoreductases in marin ...
no activity in Amphitrite sp., no activity in Cerebratulus sp., no activity in Chaetopterus variopedatus, no activity in Clymenella torquata, no activity in Glycera sp., no activity in Hydroides sp., no activity in Lepidonotus sp., no activity in Lineus sp., no activity in Nereis sp., no activity in Phascolopsis sp., no activity in Phascolosoma sp., no activity in Priapulus sp., no activity in Themiste sp., no activity in Urechis sp.
Comp. Biochem. Physiol. B
106
989-992
1993
-
-
-
-
-
-
-
-
-
-
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-
-
14
-
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-
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-
-
-
-
13292
Sato
-
Distribution of opine dehydrog ...
Anthopleura japonica, Anthopleura nigrescens, Asterias amurensis, Azumapecten farreri nipponensis, Balanus cariosus, Buccinum isaotakii, Capitulum mitella, Cellana grata, Chlorostoma lischkei, Crassostrea gigas, Fusitriton oregonensis, Halichondria japonica, Haliotis discus hannai, Liolophura japonica, Littorina brevicula, Meretrix lusoria, Mizuhopecten yessoensis, Neptunea arthritica, no activity in Aplysia juliana, no activity in Aplysia kurodai, no activity in Aurelia aurita, no activity in Halocynthia roretzi, no activity in Hemigrapsus sanguineus, no activity in Hexagrammos otakii, no activity in Loligo bleekeri, no activity in Mytilus edulis, no activity in Octopus membranaceus, no activity in Oncorhynchus keta, no activity in Pseudocardium sachalinensis, no activity in Pugettia quadridens, no activity in Stichopus japonicus, no activity in Strongylocentrotus nudus, Octopus vulgaris, Pagurus samuelis, Patiria pectinifera, Perinereis nuntia, Pseudopotamilla occelata, Reishia clavigera, Ruditapes philippinarum, Scapharca broughtonii, Solaster paxillatus, Todarodes pacificus, Tugali gigas
Comp. Biochem. Physiol. B
106
955-960
1993
-
-
-
-
-
-
-
-
-
-
-
-
-
43
-
-
-
-
-
33
-
-
29
-
-
-
-
-
-
-
-
29
-
-
-
-
-
-
29
-
-
-
-
-
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-
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-
-
-
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33
-
-
29
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13438
Sato
-
Purification and properties of ...
Rhodoglossum japonicum
Hydrobiologia
260/261
673-678
1993
-
-
-
-
-
-
-
5
-
-
1
-
-
1
-
-
1
-
-
-
1
-
3
1
1
-
1
-
2
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
5
-
-
1
-
-
-
-
1
-
-
1
-
3
1
1
-
1
-
2
-
-
-
-
-
-
-
-
-
13300
Hammen
-
Opine oxidoreductases in brach ...
Anadara ovalis, Chlorostoma funebralis, Diodora cayenensis, Glottidia pyramidata, Haliotis rufescens, Laqueus californianus, no activity in Bugula neritina, no activity in Chaetopleura apiculata, no activity in Crassostrea virginica, no activity in Crepidula fornicata, no activity in Dentalium pilsbryi, no activity in Littorina littorea, no activity in Lyonsia hyalina, no activity in Membranipora tenuis, no activity in Mopalia muscosa, no activity in Mya arenaria, no activity in Nucella lapillus, no activity in Nucula proxima, no activity in Phoronis architecta, no activity in Phoronis vancouverensis, no activity in Schizoporella floridana, no activity in Solemya velum, no activity in Spisula solidissima, no activity in Urosalpinx cinerea, Tectura testudinalis, Terebratalia transversa, Turbo castanea
Biochem. Syst. Ecol.
19
263-269
1991
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-
-
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-
-
-
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27
-
-
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-
-
-
-
-
9
-
-
-
-
-
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-
9
-
-
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-
9
-
-
-
-
-
-
-
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-
-
-
-
-
-
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-
9
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13436
Sato
-
Characterization and physiolog ...
Haliotis discus hannai
Tohoku J. Agric. Res.
41
83-95
1991
-
-
-
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9
3
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1
1
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1
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1
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1
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5
1
1
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2
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1
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1
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9
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3
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1
1
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1
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5
1
1
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13434
Gde
A specific enzymatic method fo ...
Haliotis tuberculata lamellosa
Biol. Chem. Hoppe-Seyler
368
1519-1523
1987
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3
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13435
Gde
Purification and properties of ...
Haliotis tuberculata lamellosa
Eur. J. Biochem.
160
311-318
1986
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4
7
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