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Literature summary for 1.5.1.23 extracted from

  • Kanno, N.; Sato, M.; Nagahisa, E.; Sato, Y.
    Tauropine dehydrogenase from the sandworm Arabella iricolor (polychaeta: errantia): purification and characterization (1996), Comp. Biochem. Physiol. B, 114, 409-416.
    View publication on PubMed
Search for more literature for 1.5.1.23

Inhibitors

Inhibitors Comment Organism Structure
2-oxobutanoate slight substrate inhibition Arabella iricolor
oxaloacetate slight substrate inhibition Arabella iricolor
pyruvate substrate inhibition Arabella iricolor

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.036
-
 NADH
-
 Arabella iricolor
0.051
-
 NAD+
-
 Arabella iricolor
0.34
-
 pyruvate
-
 Arabella iricolor
3.3
-
 2-oxobutanoate
-
 Arabella iricolor
4.8
-
 tauropine
-
 Arabella iricolor
4.9
-
 oxaloacetate
-
 Arabella iricolor
35.7
-
 taurine
-
 Arabella iricolor

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
43500
-
 1 * 43500, SDS-PAGE Arabella iricolor
43700
-
 gel filtration Arabella iricolor

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Arabella iricolor the enzyme is a dominant pyruvate reductase in this organism ?
-
 ?

Organism

Organism UniProt Comment Textmining
Arabella iricolor
-
-
-

Purification (Commentary)

Purification (Comment) Organism
2 enzyme forms: TaDH-1 and TaDH-2 Arabella iricolor

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
878.3
-
-
 Arabella iricolor

Storage Stability

Storage Stability Organism
4°C, 2 mM KH2PO4-NaOH, pH 7.2, 1 mM EDTA, 10 mM 2-mercaptoethanol, stable for at least 3 weeks Arabella iricolor

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Aminomethane sulfonic acid + pyruvate + NADH at 1.8% of the activity with taurine Arabella iricolor N2-(1-Carboxyethyl)aminomethanesulfonic acid + NAD+ + H2O
-
 ?
Homotaurine + pyruvate + NADH at 2.4% of the activity with taurine Arabella iricolor ?
-
 ?
Hypotaurine + pyruvate + NADH at 40% of the activity with taurine Arabella iricolor N2-(1-Carboxyethyl)aminoethanesulfinic acid + NAD+ + H2O
-
 ?
additional information the enzyme is a dominant pyruvate reductase in this organism Arabella iricolor ?
-
 ?
Taurine + 2-oxobutanoate + NADH
-
 Arabella iricolor ?
-
 ?
Taurine + 2-oxohexanoate + NADH at 4.8% of the activity with pyruvate Arabella iricolor ?
-
 ?
Taurine + 2-oxopentanoate + NADH at 7.8% of the activity with pyruvate Arabella iricolor ?
-
 ?
Taurine + 3-hydroxypyruvate + NADH at 21.2% of the activity with pyruvate Arabella iricolor ?
-
 ?
Taurine + glyoxylate + NADH at 10.9% of the activity with pyruvate Arabella iricolor ?
-
 ?
taurine + oxaloacetate + NADH
-
 Arabella iricolor ?
-
 ?
Taurine + pyruvate + NADH
-
 Arabella iricolor Tauropine + NAD+ + H2O
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 43500, SDS-PAGE Arabella iricolor

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.6 7.3 tauropine formation, enzyme form TaDH-1 Arabella iricolor
8.5
-
 tauropine oxidation Arabella iricolor

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7.9 50% of maximal activity at pH 5.5 and at pH 7.9, tauropine formation, enzyme form TaDH-1 Arabella iricolor
6.8 8.5 50% of maximal activity at pH 6.8, optimum at pH 8.5, tauropine oxidation, enzyme form TaDH-1 Arabella iricolor

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Arabella iricolor