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show all sequences of 1.5.1.22

Purification and kinetic characteristics of strombine dehydrogenase from the foot muscle of the hard clam (Meretrix lusoria)

Lee, A.C.; Lee, K.T.; Pan, L.Y.; Comp. Biochem. Physiol. B 158, 38-45 (2010)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
acetate
-
Meretrix lusoria
D-lactate
-
Meretrix lusoria
iminodiacetate
-
Meretrix lusoria
L-lactate
-
Meretrix lusoria
oxaloacetate
-
Meretrix lusoria
succinate
-
Meretrix lusoria
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.39
-
pyruvate
pH 7.5, 25C
Meretrix lusoria
34.2
-
glycine
pH 7.5, 25C
Meretrix lusoria
39.2
-
L-Ala
pH 7.5, 25C
Meretrix lusoria
45.9
-
L-2-aminobutyrate
pH 7.5, 25C
Meretrix lusoria
163
-
L-Ser
pH 7.5, 25C
Meretrix lusoria
196
-
beta-Ala
pH 7.5, 25C
Meretrix lusoria
303
-
L-Thr
pH 7.5, 25C
Meretrix lusoria
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Ca2+
10% of enzymatic activity is inhibited at 10 mM
Meretrix lusoria
Cu2+
50% of enzymatic activity is inhibited at 1 mM
Meretrix lusoria
Fe3+
inhibits enzyme at 0.2 mM
Meretrix lusoria
Mg2+
20% of enzymatic activity is inhibited at 10 mM
Meretrix lusoria
Mn2+
13% of enzymatic activity is inhibited at 1 mM
Meretrix lusoria
Zn2+
inhibits enzyme at 0.6 mM
Meretrix lusoria
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
46000
-
SDS-PAGE
Meretrix lusoria
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Meretrix lusoria
-
-
-
Purification (Commentary)
Commentary
Organism
purified over 470fold using ammonium sulfate fractionation, sephacryl S-100 chromatography, hydroxyapatite adsorption, DEAE-sepharose chromatography, Blue-sepharose chromatography
Meretrix lusoria
Source Tissue
Source Tissue
Commentary
Organism
Textmining
muscle
-
Meretrix lusoria
-
Storage Stability
Storage Stability
Organism
4C, glycerol, 2 weeks, 100% activity
Meretrix lusoria
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-Ala + pyruvate + NADH
-
711788
Meretrix lusoria
?
-
-
-
?
glycine + pyruvate + NADH
Gly and Ala are preferred substrates
711788
Meretrix lusoria
D-strombine + NAD+ + H2O
-
-
-
?
L-2-Aminobutyrate + pyruvate + NADH
-
711788
Meretrix lusoria
?
-
-
-
?
L-Ala + pyruvate + NADH
Gly and Ala are preferred substrates
711788
Meretrix lusoria
?
-
-
-
?
L-Ser + pyruvate + NADH
-
711788
Meretrix lusoria
?
-
-
-
?
L-Thr + pyruvate + NADH
-
711788
Meretrix lusoria
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
gel filtration, SDS-PAGE analysis, 1 * 46000
Meretrix lusoria
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Meretrix lusoria
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
45
46
temperature optimum
Meretrix lusoria
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
56
-
enzyme is heat labile, pretreatment at 56C for 20 sec only retains 20% of its original activity
Meretrix lusoria
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Meretrix lusoria
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7.4
7.6
pH optimum
Meretrix lusoria
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.63
-
iminodiacetate
pH 7.5, 25C
Meretrix lusoria
1.35
-
iminodiacetate
pH 6.5, 25C
Meretrix lusoria
3.69
-
acetate
pH 7.5, 25C
Meretrix lusoria
3.98
-
succinate
pH 6.5, 25C
Meretrix lusoria
5.57
-
acetate
pH 6.5, 25C
Meretrix lusoria
6.93
-
oxaloacetate
pH 7.5, 25C
Meretrix lusoria
14.3
-
oxaloacetate
pH 6.5, 25C
Meretrix lusoria
15.4
-
succinate
pH 7.5, 25C
Meretrix lusoria
16.4
-
D-lactate
pH 6.5, 25C
Meretrix lusoria
17
-
D-lactate
pH 7.5, 25C
Meretrix lusoria
21.2
-
L-lactate
pH 6.5, 25C
Meretrix lusoria
22.9
-
L-lactate
pH 7.5, 25C
Meretrix lusoria
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
acetate
-
Meretrix lusoria
D-lactate
-
Meretrix lusoria
iminodiacetate
-
Meretrix lusoria
L-lactate
-
Meretrix lusoria
oxaloacetate
-
Meretrix lusoria
succinate
-
Meretrix lusoria
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
0.63
-
iminodiacetate
pH 7.5, 25C
Meretrix lusoria
1.35
-
iminodiacetate
pH 6.5, 25C
Meretrix lusoria
3.69
-
acetate
pH 7.5, 25C
Meretrix lusoria
3.98
-
succinate
pH 6.5, 25C
Meretrix lusoria
5.57
-
acetate
pH 6.5, 25C
Meretrix lusoria
6.93
-
oxaloacetate
pH 7.5, 25C
Meretrix lusoria
14.3
-
oxaloacetate
pH 6.5, 25C
Meretrix lusoria
15.4
-
succinate
pH 7.5, 25C
Meretrix lusoria
16.4
-
D-lactate
pH 6.5, 25C
Meretrix lusoria
17
-
D-lactate
pH 7.5, 25C
Meretrix lusoria
21.2
-
L-lactate
pH 6.5, 25C
Meretrix lusoria
22.9
-
L-lactate
pH 7.5, 25C
Meretrix lusoria
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.39
-
pyruvate
pH 7.5, 25C
Meretrix lusoria
34.2
-
glycine
pH 7.5, 25C
Meretrix lusoria
39.2
-
L-Ala
pH 7.5, 25C
Meretrix lusoria
45.9
-
L-2-aminobutyrate
pH 7.5, 25C
Meretrix lusoria
163
-
L-Ser
pH 7.5, 25C
Meretrix lusoria
196
-
beta-Ala
pH 7.5, 25C
Meretrix lusoria
303
-
L-Thr
pH 7.5, 25C
Meretrix lusoria
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Ca2+
10% of enzymatic activity is inhibited at 10 mM
Meretrix lusoria
Cu2+
50% of enzymatic activity is inhibited at 1 mM
Meretrix lusoria
Fe3+
inhibits enzyme at 0.2 mM
Meretrix lusoria
Mg2+
20% of enzymatic activity is inhibited at 10 mM
Meretrix lusoria
Mn2+
13% of enzymatic activity is inhibited at 1 mM
Meretrix lusoria
Zn2+
inhibits enzyme at 0.6 mM
Meretrix lusoria
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
46000
-
SDS-PAGE
Meretrix lusoria
Purification (Commentary) (protein specific)
Commentary
Organism
purified over 470fold using ammonium sulfate fractionation, sephacryl S-100 chromatography, hydroxyapatite adsorption, DEAE-sepharose chromatography, Blue-sepharose chromatography
Meretrix lusoria
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
muscle
-
Meretrix lusoria
-
Storage Stability (protein specific)
Storage Stability
Organism
4C, glycerol, 2 weeks, 100% activity
Meretrix lusoria
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
beta-Ala + pyruvate + NADH
-
711788
Meretrix lusoria
?
-
-
-
?
glycine + pyruvate + NADH
Gly and Ala are preferred substrates
711788
Meretrix lusoria
D-strombine + NAD+ + H2O
-
-
-
?
L-2-Aminobutyrate + pyruvate + NADH
-
711788
Meretrix lusoria
?
-
-
-
?
L-Ala + pyruvate + NADH
Gly and Ala are preferred substrates
711788
Meretrix lusoria
?
-
-
-
?
L-Ser + pyruvate + NADH
-
711788
Meretrix lusoria
?
-
-
-
?
L-Thr + pyruvate + NADH
-
711788
Meretrix lusoria
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
gel filtration, SDS-PAGE analysis, 1 * 46000
Meretrix lusoria
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
25
-
assay at
Meretrix lusoria
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
45
46
temperature optimum
Meretrix lusoria
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
56
-
enzyme is heat labile, pretreatment at 56C for 20 sec only retains 20% of its original activity
Meretrix lusoria
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Meretrix lusoria
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7.4
7.6
pH optimum
Meretrix lusoria
Other publictions for EC 1.5.1.22
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743404
Murphy
Changes to coral health and m ...
Montipora capitata
PeerJ
4
e1956
2016
1
1
-
-
-
-
-
-
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1
-
2
-
-
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1
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1
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1
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1
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2
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1
1
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2
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-
1
-
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-
-
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
3
3
-
-
-
741868
Lagana
-
Anaerobiosis and metabolic pl ...
Pinna nobilis
Biochem. Syst. Ecol.
56
138-143
2014
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
5
-
-
-
-
1
-
-
-
1
-
-
2
-
-
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-
-
-
2
-
-
-
-
1
-
-
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-
-
-
-
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-
-
5
-
-
-
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
711788
Lee
Purification and kinetic chara ...
Meretrix lusoria
Comp. Biochem. Physiol. B
158
38-45
2010
-
-
-
-
-
-
6
7
-
6
1
-
-
2
-
-
1
-
-
2
-
1
6
1
1
1
1
-
1
1
-
-
12
-
-
-
-
-
-
-
-
-
-
6
12
7
-
6
1
-
-
-
-
1
-
2
-
1
6
1
1
1
1
-
1
1
-
-
-
-
-
-
-
-
703299
Plese
Strombine dehydrogenase in the ...
Suberites domuncula
Comp. Biochem. Physiol. B
154
102-107
2009
-
-
-
-
-
-
1
1
-
-
2
-
-
3
-
-
-
-
-
-
1
-
2
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
1
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
686211
Endo
-
Purification, characterization ...
Marphysa sanguinea
Comp. Biochem. Physiol. B
147B
293-307
2007
-
-
1
-
-
-
-
13
-
-
3
-
-
1
-
-
1
-
-
1
2
-
13
-
-
-
-
15
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
13
-
-
3
-
-
-
-
1
-
1
2
-
13
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
688240
Quiroga
-
Aerobic and anaerobic metaboli ...
Paraprionospio pinnata
J. Mar. Biolog. Assoc. UK
87
459-463
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13292
Sato
-
Distribution of opine dehydrog ...
Anthopleura japonica, Anthopleura nigrescens, Asterias amurensis, Azumapecten farreri nipponensis, Balanus cariosus, Buccinum isaotakii, Chlorostoma lischkei, Crassostrea gigas, Fusitriton oregonensis, Halichondria japonica, Haliotis discus hannai, Hemigrapsus sanguineus, Littorina brevicula, Meretrix lusoria, Mizuhopecten yessoensis, Mytilus edulis, Neptunea arthritica, no activity in Aplysia juliana, no activity in Aplysia kurodai, no activity in Aurelia aurita, no activity in Cellana grata, no activity in Halocynthia roretzi, no activity in Hexagrammos otakii, no activity in Liolophura japonica, no activity in Loligo bleekeri, no activity in Octopus membranaceus, no activity in Oncorhynchus keta, no activity in Pollicipes mitella, no activity in Pugettia quadridens, no activity in Solaster paxillatus, no activity in Stichopus japonicus, no activity in Strongylocentrotus nudus, no activity in Todarodes pacificus, Octopus vulgaris, Pagurus samuelis, Patiria pectinifera, Perinereis nuntia, Pseudocardium sachalinense, Pseudopotamilla occelata, Reishia clavigera, Ruditapes philippinarum, Scapharca broughtonii, Tugali gigas
Comp. Biochem. Physiol. B
106
955-960
1993
-
-
-
-
-
-
-
-
-
-
-
-
-
43
-
-
-
-
-
31
-
-
28
-
-
-
-
-
-
-
-
54
-
-
-
-
-
-
54
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31
-
-
28
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13364
Blackstock
-
Pyruvate oxidoreductase in som ...
Capitella capitata, Glycera alba, Scolelepis fuliginosa
Biochem. Soc. Trans.
15
383-384
1987
-
-
-
-
-
-
-
4
1
-
-
-
-
3
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
6
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13365
Fields
-
Tissue-specific alanopine dehy ...
Mercenaria mercenaria
J. Exp. Mar. Biol. Ecol.
105
175-185
1987
-
-
-
-
-
-
4
8
-
-
-
1
-
1
-
-
1
-
-
1
-
-
7
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
4
-
8
-
-
-
1
-
-
-
1
-
1
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13373
Storey
-
Tissue-specific alanopine dehy ...
Aphrodita aculeata
J. Exp. Zool.
225
369-378
1983
-
-
-
-
-
-
3
13
-
-
1
-
-
1
-
-
-
-
-
2
1
1
12
1
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
13
-
-
1
-
-
-
-
-
-
2
1
1
12
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
13369
Dando
-
Strombine [N-(carboxymethyl)-D ...
Mytilus edulis
Biochem. Soc. Trans.
9
297-298
1981
-
-
-
-
-
-
-
1
1
-
1
-
-
1
-
-
1
-
-
1
-
-
6
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
1
1
-
1
-
-
-
-
1
-
1
-
-
6
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-