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Literature summary for 1.5.1.22 extracted from

  • Lee, A.C.; Lee, K.T.; Pan, L.Y.
    Purification and kinetic characteristics of strombine dehydrogenase from the foot muscle of the hard clam (Meretrix lusoria) (2010), Comp. Biochem. Physiol. B, 158, 38-45.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
acetate
-
Meretrix lusoria
D-lactate
-
Meretrix lusoria
iminodiacetate
-
Meretrix lusoria
L-lactate
-
Meretrix lusoria
oxaloacetate
-
Meretrix lusoria
succinate
-
Meretrix lusoria

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.39
-
pyruvate pH 7.5, 25°C Meretrix lusoria
34.2
-
glycine pH 7.5, 25°C Meretrix lusoria
39.2
-
L-Ala pH 7.5, 25°C Meretrix lusoria
45.9
-
L-2-aminobutyrate pH 7.5, 25°C Meretrix lusoria
163
-
L-Ser pH 7.5, 25°C Meretrix lusoria
196
-
beta-Ala pH 7.5, 25°C Meretrix lusoria
303
-
L-Thr pH 7.5, 25°C Meretrix lusoria

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 10% of enzymatic activity is inhibited at 10 mM Meretrix lusoria
Cu2+ 50% of enzymatic activity is inhibited at 1 mM Meretrix lusoria
Fe3+ inhibits enzyme at 0.2 mM Meretrix lusoria
Mg2+ 20% of enzymatic activity is inhibited at 10 mM Meretrix lusoria
Mn2+ 13% of enzymatic activity is inhibited at 1 mM Meretrix lusoria
Zn2+ inhibits enzyme at 0.6 mM Meretrix lusoria

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
46000
-
SDS-PAGE Meretrix lusoria

Organism

Organism UniProt Comment Textmining
Meretrix lusoria
-
-
-

Purification (Commentary)

Purification (Comment) Organism
purified over 470fold using ammonium sulfate fractionation, sephacryl S-100 chromatography, hydroxyapatite adsorption, DEAE-sepharose chromatography, Blue-sepharose chromatography Meretrix lusoria

Source Tissue

Source Tissue Comment Organism Textmining
muscle
-
Meretrix lusoria
-

Storage Stability

Storage Stability Organism
4°C, glycerol, 2 weeks, 100% activity Meretrix lusoria

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-Ala + pyruvate + NADH
-
Meretrix lusoria ?
-
?
glycine + pyruvate + NADH Gly and Ala are preferred substrates Meretrix lusoria D-strombine + NAD+ + H2O
-
?
L-2-Aminobutyrate + pyruvate + NADH
-
Meretrix lusoria ?
-
?
L-Ala + pyruvate + NADH Gly and Ala are preferred substrates Meretrix lusoria ?
-
?
L-Ser + pyruvate + NADH
-
Meretrix lusoria ?
-
?
L-Thr + pyruvate + NADH
-
Meretrix lusoria ?
-
?

Subunits

Subunits Comment Organism
monomer gel filtration, SDS-PAGE analysis, 1 * 46000 Meretrix lusoria

Synonyms

Synonyms Comment Organism
SDH
-
Meretrix lusoria
strombine dehydrogenase
-
Meretrix lusoria

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Meretrix lusoria

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 46 temperature optimum Meretrix lusoria

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
56
-
enzyme is heat labile, pretreatment at 56°C for 20 sec only retains 20% of its original activity Meretrix lusoria

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Meretrix lusoria

pH Range

pH Minimum pH Maximum Comment Organism
7.4 7.6 pH optimum Meretrix lusoria

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.63
-
iminodiacetate pH 7.5, 25°C Meretrix lusoria
1.35
-
iminodiacetate pH 6.5, 25°C Meretrix lusoria
3.69
-
acetate pH 7.5, 25°C Meretrix lusoria
3.98
-
succinate pH 6.5, 25°C Meretrix lusoria
5.57
-
acetate pH 6.5, 25°C Meretrix lusoria
6.93
-
oxaloacetate pH 7.5, 25°C Meretrix lusoria
14.3
-
oxaloacetate pH 6.5, 25°C Meretrix lusoria
15.4
-
succinate pH 7.5, 25°C Meretrix lusoria
16.4
-
D-lactate pH 6.5, 25°C Meretrix lusoria
17
-
D-lactate pH 7.5, 25°C Meretrix lusoria
21.2
-
L-lactate pH 6.5, 25°C Meretrix lusoria
22.9
-
L-lactate pH 7.5, 25°C Meretrix lusoria