BRENDA - Enzyme Database
show all sequences of 1.5.1.22

Strombine dehydrogenase in the demosponge Suberites domuncula: characterization and kinetic properties of the enzyme crucial for anaerobic metabolism

Plese, B.; Schroeder, H.C.; Grebenjuk, V.A.; Wegener, G.; Brandt, D.; Natalio, F.; Mueller, W.E.; Comp. Biochem. Physiol. B 154, 102-107 (2009)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
pyruvate
inhibited by an excess of substrate pyruvate; substrate inhibition, in excess, pyruvate enters in competition with the other substrate (glycine and/or NADH) preventing the site of reaction to take all the substrates to proceed with the enzymatic reaction. Its optimal concentration is determined to be round 2.0-2.3 mM
Suberites domuncula
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.34
-
pyruvate
determined using constant cosubstrate concentrations (100 mM glycine and 0.3 mM NADH, pH 7.0); pH 7.0, 30°C
Suberites domuncula
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
native PAGE; semi-native PAGE
Suberites domuncula
75000
-
native PAGE; semi-native PAGE two bands at 35 kDa and 75 kDa are visible
Suberites domuncula
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Suberites domuncula
B1GT63
-
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.079
-
-
Suberites domuncula
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Gly + pyruvate + NADH
the enzyme is highly specific to glycine. No enzyme activity is observed when other amino acids (alanine and taurine) are used as substrates in the experiment
703299
Suberites domuncula
D-Strombine + NAD+ + H2O
-
-
-
?
glycine + pyruvate + NADH
enzyme is specific for glycine
703299
Suberites domuncula
D-strombine + NAD+ + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
native and semi-native PAGE analysis, 2 * 35000; native PAGE and semi-native PAGE, 2 * 35000 Da
Suberites domuncula
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Suberites domuncula
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at; assay at and optimal pH
Suberites domuncula
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
; enzyme shows an absolute requirement for NADH
Suberites domuncula
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
; enzyme shows an absolute requirement for NADH
Suberites domuncula
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
pyruvate
inhibited by an excess of substrate pyruvate; substrate inhibition, in excess, pyruvate enters in competition with the other substrate (glycine and/or NADH) preventing the site of reaction to take all the substrates to proceed with the enzymatic reaction. Its optimal concentration is determined to be round 2.0-2.3 mM
Suberites domuncula
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.34
-
pyruvate
determined using constant cosubstrate concentrations (100 mM glycine and 0.3 mM NADH, pH 7.0); pH 7.0, 30°C
Suberites domuncula
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
native PAGE; semi-native PAGE
Suberites domuncula
75000
-
native PAGE; semi-native PAGE two bands at 35 kDa and 75 kDa are visible
Suberites domuncula
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
0.079
-
-
Suberites domuncula
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Gly + pyruvate + NADH
the enzyme is highly specific to glycine. No enzyme activity is observed when other amino acids (alanine and taurine) are used as substrates in the experiment
703299
Suberites domuncula
D-Strombine + NAD+ + H2O
-
-
-
?
glycine + pyruvate + NADH
enzyme is specific for glycine
703299
Suberites domuncula
D-strombine + NAD+ + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
native and semi-native PAGE analysis, 2 * 35000; native PAGE and semi-native PAGE, 2 * 35000 Da
Suberites domuncula
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Suberites domuncula
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
-
assay at; assay at and optimal pH
Suberites domuncula
Other publictions for EC 1.5.1.22
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743404
Murphy
Changes to coral health and m ...
Montipora capitata
PeerJ
4
e1956
2016
1
1
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
1
-
-
-
1
-
-
2
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
3
3
-
-
-
741868
Lagana
-
Anaerobiosis and metabolic pl ...
Pinna nobilis
Biochem. Syst. Ecol.
56
138-143
2014
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
5
-
-
-
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
711788
Lee
Purification and kinetic chara ...
Meretrix lusoria
Comp. Biochem. Physiol. B
158
38-45
2010
-
-
-
-
-
-
6
7
-
6
1
-
-
2
-
-
1
-
-
2
-
1
6
1
1
1
1
-
1
1
-
-
12
-
-
-
-
-
-
-
-
-
-
6
12
7
-
6
1
-
-
-
-
1
-
2
-
1
6
1
1
1
1
-
1
1
-
-
-
-
-
-
-
-
703299
Plese
Strombine dehydrogenase in the ...
Suberites domuncula
Comp. Biochem. Physiol. B
154
102-107
2009
-
-
-
-
-
-
1
1
-
-
2
-
-
3
-
-
-
-
-
-
1
-
2
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
1
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
686211
Endo
-
Purification, characterization ...
Marphysa sanguinea
Comp. Biochem. Physiol. B
147B
293-307
2007
-
-
1
-
-
-
-
13
-
-
3
-
-
1
-
-
1
-
-
1
2
-
13
-
-
-
-
15
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
13
-
-
3
-
-
-
-
1
-
1
2
-
13
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
688240
Quiroga
-
Aerobic and anaerobic metaboli ...
Paraprionospio pinnata
J. Mar. Biolog. Assoc. UK
87
459-463
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13292
Sato
-
Distribution of opine dehydrog ...
Anthopleura japonica, Anthopleura nigrescens, Asterias amurensis, Azumapecten farreri nipponensis, Balanus cariosus, Buccinum isaotakii, Chlorostoma lischkei, Crassostrea gigas, Fusitriton oregonensis, Halichondria japonica, Haliotis discus hannai, Hemigrapsus sanguineus, Littorina brevicula, Meretrix lusoria, Mizuhopecten yessoensis, Mytilus edulis, Neptunea arthritica, no activity in Aplysia juliana, no activity in Aplysia kurodai, no activity in Aurelia aurita, no activity in Cellana grata, no activity in Halocynthia roretzi, no activity in Hexagrammos otakii, no activity in Liolophura japonica, no activity in Loligo bleekeri, no activity in Octopus membranaceus, no activity in Oncorhynchus keta, no activity in Pollicipes mitella, no activity in Pugettia quadridens, no activity in Solaster paxillatus, no activity in Stichopus japonicus, no activity in Strongylocentrotus nudus, no activity in Todarodes pacificus, Octopus vulgaris, Pagurus samuelis, Patiria pectinifera, Perinereis nuntia, Pseudocardium sachalinense, Pseudopotamilla occelata, Reishia clavigera, Ruditapes philippinarum, Scapharca broughtonii, Tugali gigas
Comp. Biochem. Physiol. B
106
955-960
1993
-
-
-
-
-
-
-
-
-
-
-
-
-
43
-
-
-
-
-
31
-
-
28
-
-
-
-
-
-
-
-
54
-
-
-
-
-
-
54
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31
-
-
28
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13364
Blackstock
-
Pyruvate oxidoreductase in som ...
Capitella capitata, Glycera alba, Scolelepis fuliginosa
Biochem. Soc. Trans.
15
383-384
1987
-
-
-
-
-
-
-
4
1
-
-
-
-
3
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
6
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13365
Fields
-
Tissue-specific alanopine dehy ...
Mercenaria mercenaria
J. Exp. Mar. Biol. Ecol.
105
175-185
1987
-
-
-
-
-
-
4
8
-
-
-
1
-
1
-
-
1
-
-
1
-
-
7
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
4
-
8
-
-
-
1
-
-
-
1
-
1
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13373
Storey
-
Tissue-specific alanopine dehy ...
Aphrodita aculeata
J. Exp. Zool.
225
369-378
1983
-
-
-
-
-
-
3
13
-
-
1
-
-
1
-
-
-
-
-
2
1
1
12
1
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
13
-
-
1
-
-
-
-
-
-
2
1
1
12
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
13369
Dando
-
Strombine [N-(carboxymethyl)-D ...
Mytilus edulis
Biochem. Soc. Trans.
9
297-298
1981
-
-
-
-
-
-
-
1
1
-
1
-
-
1
-
-
1
-
-
1
-
-
6
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
1
1
-
1
-
-
-
-
1
-
1
-
-
6
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-