BRENDA - Enzyme Database
show all sequences of 1.5.1.22

Purification, characterization, and cDNA cloning of opine dehydrogenases from the polychaete rockworm Marphysa sanguinea

Endo, N.; Kan-No, N.; Nagahisa, E.; Comp. Biochem. Physiol. B 147B, 293-307 (2007)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expressed in Escherichia coli
Marphysa sanguinea
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.07
-
NADH
apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C; apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
0.85
-
NAD+
apparent value, isozyme St/AlDH1, with 20 mM D-strombine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
0.87
-
NAD+
apparent value, isozyme St/AlDH1, with 20 mM meso-alanopine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
0.96
-
pyruvate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
0.97
-
pyruvate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
9.07
-
meso-Alanopine
apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C
Marphysa sanguinea
9.17
-
D-strombine
apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C
Marphysa sanguinea
10.2
-
oxaloacetate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C; apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
11.71
-
2-Ketobutyrate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
12.77
-
2-Ketobutyrate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
39.8
-
L-Ala
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
40.1
-
Gly
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
382.1
-
L-Ser
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40600
-
reduced and denatured enzyme, estimated by SDS-PAGE
Marphysa sanguinea
42000
-
native enzyme, estimated by SDS-PAGE
Marphysa sanguinea
43350
-
deduced from amino acid sequence from strombine/alanopine dehydrogenase
Marphysa sanguinea
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Marphysa sanguinea
A0A9I9
-
-
Purification (Commentary)
Commentary
Organism
ammonium sulfate precipitation, DEAE-cellulose SH column chromatography, hydroxyapatite column chromatography, and Toyopearl Super Q-650S column chromatography
Marphysa sanguinea
Source Tissue
Source Tissue
Commentary
Organism
Textmining
muscle
-
Marphysa sanguinea
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6
-
crude extract, at 30°C
Marphysa sanguinea
87
-
after 29fold purification, at 30°C
Marphysa sanguinea
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Gly + 2-ketobutyrate + NADH + H+
8.9% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
2-[(carboxymethyl)amino]butanoic acid + NAD+ + H2O
-
-
-
?
Gly + glyoxylate + NADH + H+
3.9% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
[(carboxymethyl)amino]propanedioic acid + NAD+ + H2O
-
-
-
?
Gly + oxaloacetate + NADH + H+
39.1% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
N-carboxymethylglycine + NAD+ + H2O
-
-
-
?
Gly + pyruvate + NADH + H+
94.6% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
D-strombine + NAD+ + H2O
-
-
-
?
Gly + pyruvate + NADPH
less than 0.5% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
D-strombine + NADP+ + H2O
-
-
-
?
L-Ala + 2-ketobutyrate + NADH + H+
8.9% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
2-[[(1S)-1-carboxyethyl]amino]butanoic acid + NAD+ + H2O
-
-
-
?
L-Ala + glyoxylate + NADH + H+
4.0% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
N-(carboxymethyl)-L-Ala + NAD+ + H2O
-
-
-
?
L-Ala + oxaloacetate + NADH + H+
40.0% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
[[(1S)-1-carboxyethyl]amino]propanedioic acid + NAD+ + H2O
-
-
-
?
L-Ala + pyruvate + NADH + H+
100% activity
686211
Marphysa sanguinea
meso-alanopine + NAD+ + H2O
-
-
-
?
L-Ala + pyruvate + NADPH
less than 0.5% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
meso-alanopine + NADP+ + H2O
-
-
-
?
L-Cys + pyruvate + NADH + H+
1.7% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
N-(1-carboxyethyl)-L-Cys + NAD+ + H2O
-
-
-
?
L-Ser + pyruvate + NADH + H+
14.2% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
N-(1-carboxyethyl)-L-Ser + NAD+ + H2O
-
-
-
?
L-Val + pyruvate + NADH + H+
0.9% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
N-(1-carboxyethyl)-L-Val + NAD+ + H2O
-
-
-
?
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.86
-
NAD+
apparent value, isozyme St/AlDH1, with 20 mM D-strombine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
3.96
-
NAD+
apparent value, isozyme St/AlDH1, with 20 mM meso-alanopine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
4.99
-
D-strombine
apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C
Marphysa sanguinea
5.12
-
meso-Alanopine
apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C
Marphysa sanguinea
21.77
-
2-Ketobutyrate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
23.2
-
2-Ketobutyrate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
23.64
-
L-Ser
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
42.66
-
oxaloacetate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
43.58
-
NADH
apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
44.25
-
oxaloacetate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
44.7
-
NADH
apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
47.33
-
pyruvate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
47.78
-
pyruvate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
50.08
-
Gly
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
51.7
-
L-Ala
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Marphysa sanguinea
Cloned(Commentary) (protein specific)
Commentary
Organism
expressed in Escherichia coli
Marphysa sanguinea
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Marphysa sanguinea
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.07
-
NADH
apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C; apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
0.85
-
NAD+
apparent value, isozyme St/AlDH1, with 20 mM D-strombine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
0.87
-
NAD+
apparent value, isozyme St/AlDH1, with 20 mM meso-alanopine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
0.96
-
pyruvate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
0.97
-
pyruvate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
9.07
-
meso-Alanopine
apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C
Marphysa sanguinea
9.17
-
D-strombine
apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C
Marphysa sanguinea
10.2
-
oxaloacetate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C; apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
11.71
-
2-Ketobutyrate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
12.77
-
2-Ketobutyrate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
39.8
-
L-Ala
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
40.1
-
Gly
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
382.1
-
L-Ser
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
40600
-
reduced and denatured enzyme, estimated by SDS-PAGE
Marphysa sanguinea
42000
-
native enzyme, estimated by SDS-PAGE
Marphysa sanguinea
43350
-
deduced from amino acid sequence from strombine/alanopine dehydrogenase
Marphysa sanguinea
Purification (Commentary) (protein specific)
Commentary
Organism
ammonium sulfate precipitation, DEAE-cellulose SH column chromatography, hydroxyapatite column chromatography, and Toyopearl Super Q-650S column chromatography
Marphysa sanguinea
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
muscle
-
Marphysa sanguinea
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.6
-
crude extract, at 30°C
Marphysa sanguinea
87
-
after 29fold purification, at 30°C
Marphysa sanguinea
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Gly + 2-ketobutyrate + NADH + H+
8.9% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
2-[(carboxymethyl)amino]butanoic acid + NAD+ + H2O
-
-
-
?
Gly + glyoxylate + NADH + H+
3.9% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
[(carboxymethyl)amino]propanedioic acid + NAD+ + H2O
-
-
-
?
Gly + oxaloacetate + NADH + H+
39.1% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
N-carboxymethylglycine + NAD+ + H2O
-
-
-
?
Gly + pyruvate + NADH + H+
94.6% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
D-strombine + NAD+ + H2O
-
-
-
?
Gly + pyruvate + NADPH
less than 0.5% activity compared to Gly + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
D-strombine + NADP+ + H2O
-
-
-
?
L-Ala + 2-ketobutyrate + NADH + H+
8.9% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
2-[[(1S)-1-carboxyethyl]amino]butanoic acid + NAD+ + H2O
-
-
-
?
L-Ala + glyoxylate + NADH + H+
4.0% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
N-(carboxymethyl)-L-Ala + NAD+ + H2O
-
-
-
?
L-Ala + oxaloacetate + NADH + H+
40.0% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
[[(1S)-1-carboxyethyl]amino]propanedioic acid + NAD+ + H2O
-
-
-
?
L-Ala + pyruvate + NADH + H+
100% activity
686211
Marphysa sanguinea
meso-alanopine + NAD+ + H2O
-
-
-
?
L-Ala + pyruvate + NADPH
less than 0.5% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
meso-alanopine + NADP+ + H2O
-
-
-
?
L-Cys + pyruvate + NADH + H+
1.7% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
N-(1-carboxyethyl)-L-Cys + NAD+ + H2O
-
-
-
?
L-Ser + pyruvate + NADH + H+
14.2% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
N-(1-carboxyethyl)-L-Ser + NAD+ + H2O
-
-
-
?
L-Val + pyruvate + NADH + H+
0.9% activity compared to L-Ala + pyruvate + NADH with isozyme St/AlDH1
686211
Marphysa sanguinea
N-(1-carboxyethyl)-L-Val + NAD+ + H2O
-
-
-
?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
3.86
-
NAD+
apparent value, isozyme St/AlDH1, with 20 mM D-strombine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
3.96
-
NAD+
apparent value, isozyme St/AlDH1, with 20 mM meso-alanopine, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
4.99
-
D-strombine
apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C
Marphysa sanguinea
5.12
-
meso-Alanopine
apparent value, isozyme St/AlDH1, with 1 mM NAD+, in 0.1 M PIPES-NaOH (pH 9.1), at 30°C
Marphysa sanguinea
21.77
-
2-Ketobutyrate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
23.2
-
2-Ketobutyrate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
23.64
-
L-Ser
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
42.66
-
oxaloacetate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
43.58
-
NADH
apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
44.25
-
oxaloacetate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
44.7
-
NADH
apparent value, isozyme St/AlDH1, with 5 mM pyruvate and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
47.33
-
pyruvate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM Gly, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
47.78
-
pyruvate
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 100 mM L-Ala, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
50.08
-
Gly
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
51.7
-
L-Ala
apparent value, isozyme St/AlDH1, with 0.3 mM NADH and 5 mM pyruvate, in 0.1 M PIPES-NaOH (pH 7.2), at 30°C
Marphysa sanguinea
Other publictions for EC 1.5.1.22
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743404
Murphy
Changes to coral health and m ...
Montipora capitata
PeerJ
4
e1956
2016
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1
-
1
-
-
-
1
-
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-
-
3
3
-
-
-
741868
Lagana
-
Anaerobiosis and metabolic pl ...
Pinna nobilis
Biochem. Syst. Ecol.
56
138-143
2014
-
-
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1
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1
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5
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1
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1
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2
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2
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1
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5
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1
-
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-
1
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-
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2
2
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-
711788
Lee
Purification and kinetic chara ...
Meretrix lusoria
Comp. Biochem. Physiol. B
158
38-45
2010
-
-
-
-
-
-
6
7
-
6
1
-
-
2
-
-
1
-
-
2
-
1
6
1
1
1
1
-
1
1
-
-
12
-
-
-
-
-
-
-
-
-
-
6
12
7
-
6
1
-
-
-
-
1
-
2
-
1
6
1
1
1
1
-
1
1
-
-
-
-
-
-
-
-
703299
Plese
Strombine dehydrogenase in the ...
Suberites domuncula
Comp. Biochem. Physiol. B
154
102-107
2009
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-
-
-
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1
1
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2
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3
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1
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2
1
1
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-
1
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-
1
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-
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-
-
1
-
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
1
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
686211
Endo
-
Purification, characterization ...
Marphysa sanguinea
Comp. Biochem. Physiol. B
147B
293-307
2007
-
-
1
-
-
-
-
13
-
-
3
-
-
1
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-
1
-
-
1
2
-
13
-
-
-
-
15
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
13
-
-
3
-
-
-
-
1
-
1
2
-
13
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
688240
Quiroga
-
Aerobic and anaerobic metaboli ...
Paraprionospio pinnata
J. Mar. Biolog. Assoc. UK
87
459-463
2007
-
-
-
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1
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-
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1
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-
-
-
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-
1
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-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13292
Sato
-
Distribution of opine dehydrog ...
Anthopleura japonica, Anthopleura nigrescens, Asterias amurensis, Azumapecten farreri nipponensis, Balanus cariosus, Buccinum isaotakii, Chlorostoma lischkei, Crassostrea gigas, Fusitriton oregonensis, Halichondria japonica, Haliotis discus hannai, Hemigrapsus sanguineus, Littorina brevicula, Meretrix lusoria, Mizuhopecten yessoensis, Mytilus edulis, Neptunea arthritica, no activity in Aplysia juliana, no activity in Aplysia kurodai, no activity in Aurelia aurita, no activity in Cellana grata, no activity in Halocynthia roretzi, no activity in Hexagrammos otakii, no activity in Liolophura japonica, no activity in Loligo bleekeri, no activity in Octopus membranaceus, no activity in Oncorhynchus keta, no activity in Pollicipes mitella, no activity in Pugettia quadridens, no activity in Solaster paxillatus, no activity in Stichopus japonicus, no activity in Strongylocentrotus nudus, no activity in Todarodes pacificus, Octopus vulgaris, Pagurus samuelis, Patiria pectinifera, Perinereis nuntia, Pseudocardium sachalinense, Pseudopotamilla occelata, Reishia clavigera, Ruditapes philippinarum, Scapharca broughtonii, Tugali gigas
Comp. Biochem. Physiol. B
106
955-960
1993
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43
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31
-
-
28
-
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-
-
-
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-
54
-
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-
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-
54
-
-
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-
-
-
-
-
-
-
-
-
-
-
-
31
-
-
28
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13364
Blackstock
-
Pyruvate oxidoreductase in som ...
Capitella capitata, Glycera alba, Scolelepis fuliginosa
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15
383-384
1987
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4
1
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3
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1
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3
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-
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6
-
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-
6
-
-
-
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-
4
1
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-
-
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-
1
-
-
3
-
-
-
-
-
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-
-
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-
-
-
-
-
-
13365
Fields
-
Tissue-specific alanopine dehy ...
Mercenaria mercenaria
J. Exp. Mar. Biol. Ecol.
105
175-185
1987
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4
8
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1
-
1
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1
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-
1
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7
-
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-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
4
-
8
-
-
-
1
-
-
-
1
-
1
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13373
Storey
-
Tissue-specific alanopine dehy ...
Aphrodita aculeata
J. Exp. Zool.
225
369-378
1983
-
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3
13
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1
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1
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2
1
1
12
1
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-
-
-
2
-
-
2
-
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-
-
-
-
2
-
-
-
-
3
-
13
-
-
1
-
-
-
-
-
-
2
1
1
12
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
13369
Dando
-
Strombine [N-(carboxymethyl)-D ...
Mytilus edulis
Biochem. Soc. Trans.
9
297-298
1981
-
-
-
-
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1
1
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1
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1
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1
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1
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6
-
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-
2
-
-
2
-
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-
-
2
-
-
-
-
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1
1
-
1
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1
-
1
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6
-
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2
-
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-