BRENDA - Enzyme Database
show all sequences of 1.5.1.22

Tissue-specific alanopine dehydrogenase from the gill and strombine dehydrogenase from the foot muscle of the cherrystone clam Mercenaria mercenaria

Fields, J.H.A.; Storey, K.B.; J. Exp. Mar. Biol. Ecol. 105, 175-185 (1987)
No PubMed abstract available

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
D-lactate
weak
Mercenaria mercenaria
L-lactate
weak
Mercenaria mercenaria
meso-Alanopine
weak
Mercenaria mercenaria
succinate
weak
Mercenaria mercenaria
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.32
-
pyruvate
-
Mercenaria mercenaria
0.61
-
pyruvate
-
Mercenaria mercenaria
2.46
-
2-oxobutanoate
-
Mercenaria mercenaria
4.18
-
glyoxylate
-
Mercenaria mercenaria
44
-
L-2-aminobutanoate
-
Mercenaria mercenaria
173
-
L-Ala
-
Mercenaria mercenaria
242
-
L-Ala
-
Mercenaria mercenaria
693
-
L-Ser
-
Mercenaria mercenaria
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Mercenaria mercenaria
the enzyme functions as the terminal dehydrogenase of glycolysis and has a role in maintaining energy production under the stresses of environmental or functional anoxia
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Mercenaria mercenaria
-
-
-
Purification (Commentary)
Commentary
Organism
partial
Mercenaria mercenaria
Source Tissue
Source Tissue
Commentary
Organism
Textmining
foot muscle
-
Mercenaria mercenaria
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Gly + 2-oxobutanoate + NADH
30% of the activity with pyruvate
13365
Mercenaria mercenaria
?
-
-
-
-
Gly + glyoxylate + NADH
20% of the activity with pyruvate
13365
Mercenaria mercenaria
?
-
-
-
-
Gly + pyruvate + NADH
-
13365
Mercenaria mercenaria
D-Strombine + NAD+ + H2O
-
-
-
-
L-2-Aminobutanoate + pyruvate + NADH
at 48% of the activity with Gly
13365
Mercenaria mercenaria
?
-
-
-
-
L-Ala + pyruvate + NADH
at 76% of the activity with Gly
13365
Mercenaria mercenaria
meso-Alanopine + NAD+ + H2O
-
-
-
-
L-Ser + pyruvate + NADH
at 82% of the activity with Gly
13365
Mercenaria mercenaria
?
-
-
-
-
additional information
the enzyme functions as the terminal dehydrogenase of glycolysis and has a role in maintaining energy production under the stresses of environmental or functional anoxia
13365
Mercenaria mercenaria
?
-
-
-
-
Cofactor
Cofactor
Commentary
Organism
Structure
NAD+
-
Mercenaria mercenaria
NADH
-
Mercenaria mercenaria
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NAD+
-
Mercenaria mercenaria
NADH
-
Mercenaria mercenaria
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
D-lactate
weak
Mercenaria mercenaria
L-lactate
weak
Mercenaria mercenaria
meso-Alanopine
weak
Mercenaria mercenaria
succinate
weak
Mercenaria mercenaria
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.32
-
pyruvate
-
Mercenaria mercenaria
0.61
-
pyruvate
-
Mercenaria mercenaria
2.46
-
2-oxobutanoate
-
Mercenaria mercenaria
4.18
-
glyoxylate
-
Mercenaria mercenaria
44
-
L-2-aminobutanoate
-
Mercenaria mercenaria
173
-
L-Ala
-
Mercenaria mercenaria
242
-
L-Ala
-
Mercenaria mercenaria
693
-
L-Ser
-
Mercenaria mercenaria
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Mercenaria mercenaria
the enzyme functions as the terminal dehydrogenase of glycolysis and has a role in maintaining energy production under the stresses of environmental or functional anoxia
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
partial
Mercenaria mercenaria
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
foot muscle
-
Mercenaria mercenaria
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Gly + 2-oxobutanoate + NADH
30% of the activity with pyruvate
13365
Mercenaria mercenaria
?
-
-
-
-
Gly + glyoxylate + NADH
20% of the activity with pyruvate
13365
Mercenaria mercenaria
?
-
-
-
-
Gly + pyruvate + NADH
-
13365
Mercenaria mercenaria
D-Strombine + NAD+ + H2O
-
-
-
-
L-2-Aminobutanoate + pyruvate + NADH
at 48% of the activity with Gly
13365
Mercenaria mercenaria
?
-
-
-
-
L-Ala + pyruvate + NADH
at 76% of the activity with Gly
13365
Mercenaria mercenaria
meso-Alanopine + NAD+ + H2O
-
-
-
-
L-Ser + pyruvate + NADH
at 82% of the activity with Gly
13365
Mercenaria mercenaria
?
-
-
-
-
additional information
the enzyme functions as the terminal dehydrogenase of glycolysis and has a role in maintaining energy production under the stresses of environmental or functional anoxia
13365
Mercenaria mercenaria
?
-
-
-
-
Other publictions for EC 1.5.1.22
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
743404
Murphy
Changes to coral health and m ...
Montipora capitata
PeerJ
4
e1956
2016
1
1
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
1
-
-
1
-
1
-
-
-
1
-
-
2
-
-
-
1
1
-
2
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
3
3
-
-
-
741868
Lagana
-
Anaerobiosis and metabolic pl ...
Pinna nobilis
Biochem. Syst. Ecol.
56
138-143
2014
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
5
-
-
-
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
711788
Lee
Purification and kinetic chara ...
Meretrix lusoria
Comp. Biochem. Physiol. B
158
38-45
2010
-
-
-
-
-
-
6
7
-
6
1
-
-
2
-
-
1
-
-
2
-
1
6
1
1
1
1
-
1
1
-
-
12
-
-
-
-
-
-
-
-
-
-
6
12
7
-
6
1
-
-
-
-
1
-
2
-
1
6
1
1
1
1
-
1
1
-
-
-
-
-
-
-
-
703299
Plese
Strombine dehydrogenase in the ...
Suberites domuncula
Comp. Biochem. Physiol. B
154
102-107
2009
-
-
-
-
-
-
1
1
-
-
2
-
-
3
-
-
-
-
-
-
1
-
2
1
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
1
-
2
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
686211
Endo
-
Purification, characterization ...
Marphysa sanguinea
Comp. Biochem. Physiol. B
147B
293-307
2007
-
-
1
-
-
-
-
13
-
-
3
-
-
1
-
-
1
-
-
1
2
-
13
-
-
-
-
15
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
-
-
13
-
-
3
-
-
-
-
1
-
1
2
-
13
-
-
-
-
15
-
-
-
-
-
-
-
-
-
-
688240
Quiroga
-
Aerobic and anaerobic metaboli ...
Paraprionospio pinnata
J. Mar. Biolog. Assoc. UK
87
459-463
2007
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13292
Sato
-
Distribution of opine dehydrog ...
Anthopleura japonica, Anthopleura nigrescens, Asterias amurensis, Azumapecten farreri nipponensis, Balanus cariosus, Buccinum isaotakii, Chlorostoma lischkei, Crassostrea gigas, Fusitriton oregonensis, Halichondria japonica, Haliotis discus hannai, Hemigrapsus sanguineus, Littorina brevicula, Meretrix lusoria, Mizuhopecten yessoensis, Mytilus edulis, Neptunea arthritica, no activity in Aplysia juliana, no activity in Aplysia kurodai, no activity in Aurelia aurita, no activity in Cellana grata, no activity in Halocynthia roretzi, no activity in Hexagrammos otakii, no activity in Liolophura japonica, no activity in Loligo bleekeri, no activity in Octopus membranaceus, no activity in Oncorhynchus keta, no activity in Pollicipes mitella, no activity in Pugettia quadridens, no activity in Solaster paxillatus, no activity in Stichopus japonicus, no activity in Strongylocentrotus nudus, no activity in Todarodes pacificus, Octopus vulgaris, Pagurus samuelis, Patiria pectinifera, Perinereis nuntia, Pseudocardium sachalinense, Pseudopotamilla occelata, Reishia clavigera, Ruditapes philippinarum, Scapharca broughtonii, Tugali gigas
Comp. Biochem. Physiol. B
106
955-960
1993
-
-
-
-
-
-
-
-
-
-
-
-
-
43
-
-
-
-
-
31
-
-
28
-
-
-
-
-
-
-
-
54
-
-
-
-
-
-
54
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
31
-
-
28
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13364
Blackstock
-
Pyruvate oxidoreductase in som ...
Capitella capitata, Glycera alba, Scolelepis fuliginosa
Biochem. Soc. Trans.
15
383-384
1987
-
-
-
-
-
-
-
4
1
-
-
-
-
3
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
6
-
-
-
-
-
-
4
1
-
-
-
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13365
Fields
-
Tissue-specific alanopine dehy ...
Mercenaria mercenaria
J. Exp. Mar. Biol. Ecol.
105
175-185
1987
-
-
-
-
-
-
4
8
-
-
-
1
-
1
-
-
1
-
-
1
-
-
7
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
4
-
8
-
-
-
1
-
-
-
1
-
1
-
-
7
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
13373
Storey
-
Tissue-specific alanopine dehy ...
Aphrodita aculeata
J. Exp. Zool.
225
369-378
1983
-
-
-
-
-
-
3
13
-
-
1
-
-
1
-
-
-
-
-
2
1
1
12
1
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
3
-
13
-
-
1
-
-
-
-
-
-
2
1
1
12
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
13369
Dando
-
Strombine [N-(carboxymethyl)-D ...
Mytilus edulis
Biochem. Soc. Trans.
9
297-298
1981
-
-
-
-
-
-
-
1
1
-
1
-
-
1
-
-
1
-
-
1
-
-
6
-
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
1
1
-
1
-
-
-
-
1
-
1
-
-
6
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-